FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_48.3510000004f657.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_48.3510000004f657.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2842
Sequences flagged as poor quality0
Sequence length46
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATACTGTAGGCATGGGTGATGCTGGTATTAAATCTGCCATTCA38713.617171006333567No Hit
AACGGTCACATTAAATTTAACCTGACTATTCCACTGCAACAACTGA36712.91344123856439No Hit
CCACTGACCCTCAGCAATCTTAAACTTCTTAGACGAATCACCAGAA31511.083743842364532No Hit
ATGGTGATATGTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCG31210.978184377199156No Hit
CGCTCAAAGTCAAAATAATCAGCGTGACATTCAGAAGGGTAATAAG2589.078114004222378No Hit
ACCGGAGTAGTTGAAATGGTAATAAGACGACCAATCTGACCAGCAA2358.268824771287825No Hit
AAAATGATTGGCGTATCCAACCTGCAGAGTTTTATCGCTTCCATGA2247.8817733990147785No Hit
CTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTC1946.826178747361013No Hit
TTGCTGGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGAAAT1806.33356790992259No Hit
AACCAACCATCAGCATGAGCCTGTCGCATTGCATTCATCAAACGCT1374.82054890921886No Hit
AAACTCTGCAGGTTGGATACGCCAATCATTTTTATCGAAGCGCGCA1314.609429978888107No Hit
CTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCCC50.17593244194229415No Hit
CTTGTAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTC30.1055594651653765No Hit
ACCGAAGTAGTTGAAATGGTAATAAGACGACCAATCTGACCAGCAA30.1055594651653765No Hit
AACGATCACATTAAATTTAACCTGACTATTCCACTGCAACAACTGA30.1055594651653765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTA303.3510933E-640.016
ATGGGTG351.8523497E-740.015
TGATGCT351.8523497E-740.020
GTATGTT351.8523497E-740.011
GGTGATG351.8523497E-740.018
GGTGATA351.8523497E-740.03
TGTTGAC351.8523497E-740.014
GTATGGC303.3510933E-640.020
ACTGCAA351.8523497E-740.033
ACTGTAG351.8523497E-740.06
ACCGGAG351.8523497E-740.01
GTTGACG351.8523497E-740.015
GTTGAAA351.8523497E-740.010
CGGAGTA351.8523497E-740.03
CCGGAGT351.8523497E-740.02
CCAACCT401.0086296E-840.017
TAAATTT351.8523497E-740.012
CCAATCT351.8523497E-740.031
AATCTGC351.8523497E-740.034
AATCTGA351.8523497E-740.033
Produced by FastQC (version 0.11.2)