000000000-AKRU9_l01n01_exp163gap1bff_47.3510000004f64a.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_47.3510000004f64a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197282
Sequences flagged as poor quality	0
Sequence length	46
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	28404	14.39766425725611	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	2324	1.178009144270638	No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	903	0.45772042051479606	No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA	720	0.36495980373272774	RNA PCR Primer, Index 41 (96% over 28bp)
AAGAAAAGCGGCATGGTCAATATAACCAGTAGTGTTAACAGTCGGG	548	0.27777496172990945	No Hit
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA	521	0.2640889690899322	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGGAAGGGAATTTGGGTGTGGCTGCAGGTCGACATCTC	370	0.18754878802931843	No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG	348	0.17639723847081842	No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA	344	0.1743696840056366	No Hit
GTCCACGAGGTCTCTAGAGGTATAACACTCACCCCGTACGCTGCAG	297	0.1505459190397502	No Hit
GTCCACGAGGTCTCTGCGGCTGCAATACAGTTCTACGTACGCTGCA	286	0.1449701442605002	No Hit
GTCCACGAGGTCTCTTACGCGAGTGCATGTGCCATCGTACGCTGCA	273	0.13838059224865928	No Hit
GTCCACGAGGTCTCTCGACTAGCATGACATTTCAGCGTACGCTGCA	262	0.13280481746940928	No Hit
GTCCACGAGGTCTCTGAGATGTGGATCTCACCCATCGTACGCTGCA	259	0.1312841516205229	No Hit
ATGCTCAGGAACAAAGAAACGCGGCACAGAATGTTTATAGGTCTGT	258	0.13077726300422746	No Hit
GTCCACGAGGTCTCTAACATACGAGGCGGTGCAATCGTACGCTGCA	258	0.13077726300422746	No Hit
GTCCACGAGGTCTCTAGCGAGCACACATTCATTACCGTACGCTGCA	245	0.12418771099238653	No Hit
AATTCATCCATTAACTTCTCAGTAACAGATACAAACTCATCACGAA	242	0.12266704514350016	No Hit
GTCCACGAGGTCTCTCCTAGAGGCTAATTTGCATCCGTACGCTGCA	242	0.12266704514350016	No Hit
GTCCACGAGGTCTCTGACTTACTCGTACCCGATTTCGTACGCTGCA	238	0.12063949067831835	No Hit
GTCCACGAGGTCTCTAGACGGACTCGCATTCACTGCGTACGCTGCA	238	0.12063949067831835	No Hit
GTCCACGAGGTCTCTACTACTGGCTTAGATGTGGTCGTACGCTGCA	238	0.12063949067831835	No Hit
GTCCACGAGGTCTCTATATGACCTGAACCGACGGCCGTACGCTGCA	233	0.11810504759684107	No Hit
GTCCACGAGGTCTCTCACTCATGGTTGGGAATTAGCGTACGCTGCA	232	0.11759815898054563	No Hit
AAGCATATTAAGCCACTTCTCCTCATCCAACGCGTCAGTTTTTGAC	231	0.11709127036425016	No Hit
GTCCACGAGGTCTCTATACTTGTGCAACGTGCGCCCGTACGCTGCA	229	0.11607749313165924	No Hit
GTCCACGAGGTCTCTGCGACATACACACAGCGGTACATACGCTGCA	227	0.11506371589906834	No Hit
GTCCACGAGGTCTCTAGCTATTACCCATTCTACGCGTACGCTGCAG	224	0.11354305005018196	No Hit
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA	224	0.11354305005018196	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA	218	0.11050171835240924	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCCATCGGTGTCATGTACTTCGTACGCTGCA	215	0.10898105250352287	No Hit
GTCCACGAGGTCTCTGAGTATATCATCTCACACCGCGTACGCTGCA	212	0.10746038665463652	No Hit
GTCCACGAGGTCTCTGCAGATAATACACTGTCACCCGTACGCTGCA	210	0.10644660942204559	No Hit
TGAGTATGGTACAGCTAATGGCCGTCTTCATTTCCATGCGGTGCAC	208	0.10543283218945468	No Hit
GTCCACGAGGTCTCTCTGAGAAGCGTGCATTAGATCGTACGCTGCA	207	0.10492594357315924	No Hit
GTCCACGAGGTCTCTCGTCTGACATAAGAGCAACCGTACGCTGCAG	206	0.10441905495686378	No Hit
AGCCTGCAACGTACCTTCAAGAAGTCCTTTACCAGCTTTAGCCATA	202	0.10239150049168196	No Hit
GTCCACGAGGTCTCTTTCCATGCCGGACGTAGGATCGTACGCTGCA	201	0.1018846118753865	No Hit
GTCCACGAGGTCTCTATGATACTAGCATCGAGGCGCGTACGCTGCA	201	0.1018846118753865	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAA	30	4.8470065E-6	40.000004	15
GTTTGTT	30	4.8470065E-6	40.000004	24
ATCCTCC	30	4.8470065E-6	40.000004	30
TGTTTAT	30	4.8470065E-6	40.000004	32
CGTGGTA	30	4.8470065E-6	40.000004	19
CAGAATG	30	4.8470065E-6	40.000004	27
TAGAGTC	30	4.8470065E-6	40.000004	30
AAACTGA	30	4.8470065E-6	40.000004	25
TCTGATC	120	0.0	40.000004	13
GAGTGAA	30	4.8470065E-6	40.000004	21
AGCCGCA	30	4.8470065E-6	40.000004	21
TCTGACT	135	0.0	40.000004	13
TCTGACC	120	0.0	40.000004	13
TCTGAAA	30	4.8470065E-6	40.000004	13
GCCTTCG	30	4.8470065E-6	40.000004	31
ATCTCCG	30	4.8470065E-6	40.000004	31
AGTCCAT	30	4.8470065E-6	40.000004	22
CAAACCG	30	4.8470065E-6	40.000004	31
CAAACAA	30	4.8470065E-6	40.000004	20
ACCGGCT	30	4.8470065E-6	40.000004	20

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content