FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_46.3510000004f63d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_46.3510000004f63d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379250
Sequences flagged as poor quality0
Sequence length46
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA9609425.33790375741595Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC33110.8730388925510877No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA26190.6905735003295979RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA17050.4495715227422544No Hit
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA9590.25286750164798943RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA8450.2228081740276862RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA6890.18167435728411338Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA5440.14344100197758736No Hit
ACATAGAAACCAACAGCCATATAACTGGTAGCTTTAAGCGGCTCAC5260.13869479235332896No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGCAAAAAA4990.13157547791694132Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTAGATGCTCATAACGCTGCTCCGTACGCTGCA4300.1133816743572841No Hit
GTCCACGAGGTCTCTAGAGTGTCCCAAGTTCCCTTCGTACGCTGCA4100.10810810810810811No Hit
GTCCACGAGGTCTCTGGTGCTCAGTCATCTTCACTCGTACGCTGCA3940.10388925510876731No Hit
GTCCACGAGGTCTCTCGATCCACAGTAGCTGAATCCGTACGCTGCA3840.1012524719841793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTATT304.858879E-640.00000427
GTTTGTT304.858879E-640.00000424
CTTGTAT304.858879E-640.00000414
TCGCCGT600.040.00000432
CTTAAGC304.858879E-640.00000414
ACAACGA304.858879E-640.00000424
GTTGAAA304.858879E-640.00000421
GGAATTT304.858879E-640.00000415
GAGGATA304.858879E-640.00000423
GTGCACG304.858879E-640.00000431
CATGGGA304.858879E-640.00000426
AAACTCT304.858879E-640.00000425
GGGTGTG304.858879E-640.00000422
ATGGGAG304.858879E-640.00000427
GAGGAAC304.858879E-640.00000417
CGGAAAT304.858879E-640.00000425
ACAGTTG304.858879E-640.00000425
TGATACT600.040.00000415
AATCCGG304.858879E-640.00000426
GAAGCTT304.858879E-640.00000426