Basic Statistics
Measure | Value |
---|---|
Filename | 000000000-AKRU9_l01n01_exp163gap1bff_46.3510000004f63d.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 379250 |
Sequences flagged as poor quality | 0 |
Sequence length | 46 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 96094 | 25.33790375741595 | Illumina PCR Primer Index 11 (96% over 28bp) |
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC | 3311 | 0.8730388925510877 | No Hit |
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 2619 | 0.6905735003295979 | RNA PCR Primer, Index 41 (96% over 28bp) |
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA | 1705 | 0.4495715227422544 | No Hit |
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA | 959 | 0.25286750164798943 | RNA PCR Primer, Index 41 (96% over 30bp) |
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA | 845 | 0.2228081740276862 | RNA PCR Primer, Index 41 (96% over 28bp) |
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA | 689 | 0.18167435728411338 | Illumina PCR Primer Index 11 (96% over 28bp) |
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA | 544 | 0.14344100197758736 | No Hit |
ACATAGAAACCAACAGCCATATAACTGGTAGCTTTAAGCGGCTCAC | 526 | 0.13869479235332896 | No Hit |
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGCAAAAAA | 499 | 0.13157547791694132 | Illumina PCR Primer Index 11 (96% over 28bp) |
GTCCACGAGGTCTCTAGATGCTCATAACGCTGCTCCGTACGCTGCA | 430 | 0.1133816743572841 | No Hit |
GTCCACGAGGTCTCTAGAGTGTCCCAAGTTCCCTTCGTACGCTGCA | 410 | 0.10810810810810811 | No Hit |
GTCCACGAGGTCTCTGGTGCTCAGTCATCTTCACTCGTACGCTGCA | 394 | 0.10388925510876731 | No Hit |
GTCCACGAGGTCTCTCGATCCACAGTAGCTGAATCCGTACGCTGCA | 384 | 0.1012524719841793 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATTTATT | 30 | 4.858879E-6 | 40.000004 | 27 |
GTTTGTT | 30 | 4.858879E-6 | 40.000004 | 24 |
CTTGTAT | 30 | 4.858879E-6 | 40.000004 | 14 |
TCGCCGT | 60 | 0.0 | 40.000004 | 32 |
CTTAAGC | 30 | 4.858879E-6 | 40.000004 | 14 |
ACAACGA | 30 | 4.858879E-6 | 40.000004 | 24 |
GTTGAAA | 30 | 4.858879E-6 | 40.000004 | 21 |
GGAATTT | 30 | 4.858879E-6 | 40.000004 | 15 |
GAGGATA | 30 | 4.858879E-6 | 40.000004 | 23 |
GTGCACG | 30 | 4.858879E-6 | 40.000004 | 31 |
CATGGGA | 30 | 4.858879E-6 | 40.000004 | 26 |
AAACTCT | 30 | 4.858879E-6 | 40.000004 | 25 |
GGGTGTG | 30 | 4.858879E-6 | 40.000004 | 22 |
ATGGGAG | 30 | 4.858879E-6 | 40.000004 | 27 |
GAGGAAC | 30 | 4.858879E-6 | 40.000004 | 17 |
CGGAAAT | 30 | 4.858879E-6 | 40.000004 | 25 |
ACAGTTG | 30 | 4.858879E-6 | 40.000004 | 25 |
TGATACT | 60 | 0.0 | 40.000004 | 15 |
AATCCGG | 30 | 4.858879E-6 | 40.000004 | 26 |
GAAGCTT | 30 | 4.858879E-6 | 40.000004 | 26 |