000000000-AKRU9_l01n01_exp163gap1bff_44.3510000004f613.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_44.3510000004f613.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269253
Sequences flagged as poor quality	0
Sequence length	46
%GC	51

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	132690	49.280787957794345	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA	3381	1.2556963153613887	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	1577	0.5856944955116563	No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA	1070	0.39739575789313397	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA	834	0.3097458524139007	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	702	0.26072132901026174	No Hit
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA	690	0.25626455415538546	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGCAAAAAA	663	0.24623681073191384	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCGACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	382	0.14187399954689456	RNA PCR Primer, Index 41 (96% over 28bp)
AGAGGGCGTTCAGCAGCCAGCTTGCGGCAAAACTGCGTAACCGTCT	309	0.11476195251306391	No Hit
TGGTTACAGTATGCCCATCGCAGTTCGCTACACGCAGGACGCTTTT	294	0.10919098394446859	No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA	287	0.10659119861245743	No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCGTGAAAAAAA	284	0.10547700489873836	RNA PCR Primer, Index 41 (96% over 28bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAA	30	4.8536203E-6	40.000004	15
TCGCCAG	30	4.8536203E-6	40.000004	15
TGTTTCC	30	4.8536203E-6	40.000004	30
AAACTGC	30	4.8536203E-6	40.000004	30
CTATGAG	30	4.8536203E-6	40.000004	14
TCTTGTC	60	0.0	40.000004	13
CTGTCCG	30	4.8536203E-6	40.000004	31
GTCGCGT	30	4.8536203E-6	40.000004	32
CTCAAAT	30	4.8536203E-6	40.000004	22
CAAAACT	30	4.8536203E-6	40.000004	28
GTTTCCG	30	4.8536203E-6	40.000004	31
CAAAAAA	120	0.0	40.000004	40
TCTTCTA	30	4.8536203E-6	40.000004	13
TCACAAT	30	4.8536203E-6	40.000004	23
TTGCTTG	30	4.8536203E-6	40.000004	33
AACTTTC	30	4.8536203E-6	40.000004	26
GGCGTTC	30	4.8536203E-6	40.000004	5
CGGCATC	30	4.8536203E-6	40.000004	19
AGCTTGC	30	4.8536203E-6	40.000004	19
CAGCAGC	30	4.8536203E-6	40.000004	11