FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_43.3510000004f606.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_43.3510000004f606.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336922
Sequences flagged as poor quality0
Sequence length46
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA5178815.370916710692683Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC42451.2599355340405198No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA12720.37753545330966815No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA12610.37427060269142415RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA12200.36210161402342383RNA PCR Primer, Index 41 (96% over 30bp)
GCGTGTAGCGAACTGCGATGGGCATACTGTAACCATAAGGCCACGT7170.21280889938917613No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA5550.16472655392049199No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA3890.11545699004517367No Hit
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA3760.11159853022361257RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA3630.10774007040205151No Hit
TCCCCGTACGCCGGGCAATAATGTTTATGTTGGTTTCATGGTTTGG3520.10447521978380753No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA3440.10210078297053918Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG3390.10061675996224646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA257.78291E-540.00000415
ATTTACC257.78291E-540.00000421
TTGATAT257.78291E-540.00000429
CTTAAAC257.78291E-540.00000422
TTAGGTA304.85726E-640.00000415
AAACTCT304.85726E-640.00000425
TGATATA304.85726E-640.00000415
AGCGTCA304.85726E-640.00000427
AACAAGC304.85726E-640.00000424
AGCAAGG257.78291E-540.00000427
CGGCGGA257.78291E-540.00000417
CGGCGCT257.78291E-540.00000416
GGCAGGG257.78291E-540.00000421
TGATAAA304.85726E-640.00000427
CGGCGAG257.78291E-540.00000422
CTGTCCT257.78291E-540.00000414
TTGGCCA304.85726E-640.00000423
AATCCAC257.78291E-540.00000429
GGTACCG257.78291E-540.00000431
AAATCGG304.85726E-640.00000425
Produced by FastQC (version 0.11.2)