FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_42.3510000004f5f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_42.3510000004f5f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270927
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA53281.9665814038467926Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC36271.338737002956516No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA11050.4078589435530604No Hit
CTGATGCTTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAATCA4720.1742166709113525No Hit
CTATGGCTAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGC3440.12697147202013825No Hit
GCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCCTCCGG3330.12291133774042454No Hit
GTCCACGAGGTCTCTAGTCGGATCACCCGCTGAATCGTACGCTGCA3200.1181129972280356No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA3140.11589837853000992No Hit
GTCCACGAGGTCTCTAGAGTGTCCCAAGTTCCCTTCGTACGCTGCA3050.11257645048297144No Hit
GTCCACGAGGTCTCTATGACGTGTCGATCACTCCTCGTACGCTGCA2900.10703990373790725No Hit
GTCCACGAGGTCTCTGGTATGACTATCACCCATCGCGTACGCTGCA2870.10593259438889444No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG2850.1051943881562192No Hit
CTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTC2750.10150335699284309No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAG200.001254070640.00000428
TCCGGAT200.001254070640.00000415
TATTTTT200.001254070640.0000048
GTTGATA200.001254070640.0000043
CTTGTGG401.9190338E-840.00000414
GTTTGTT401.9190338E-840.00000424
TATTTCT200.001254070640.00000416
GTGTTCA200.001254070640.00000422
CTTAAAC401.9190338E-840.00000422
TTGATAA200.001254070640.00000422
TGTTTCC200.001254070640.00000430
TGTTTAC200.001254070640.00000430
GAGGATA200.001254070640.00000423
GAAATGA401.9190338E-840.00000424
ACTATTA200.001254070640.00000421
AGCAATT200.001254070640.00000423
AAACTCT200.001254070640.00000425
CTATGGT200.001254070640.00000414
GAAATAG200.001254070640.00000424
AACAAGC200.001254070640.00000424
Produced by FastQC (version 0.11.2)