FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_41.3510000004f5eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_41.3510000004f5eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416954
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC80441.9292296032655882No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA26870.6444355972121625Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA16260.3899710759460276No Hit
GTCCACGAGGTCTCTATGACGTGTCGATCACTCCTCGTACGCTGCA5270.12639283949788224No Hit
GTCCACGAGGTCTCTGGCCCGCACACAATTAGGAACGTACGCTGCA4580.10984425140423164No Hit
GTCCACGAGGTCTCTCAGACGAGCTAATTCTATCCCGTACGCTGCA4400.1055272284232793No Hit
GTCCACGAGGTCTCTAGACGGACTCGCATTCACTGCGTACGCTGCA4350.10432805537301476No Hit
GTCCACGAGGTCTCTCACGGTTATCAGATGTGGATCGTACGCTGCA4320.10360855154285604No Hit
GTCCACGAGGTCTCTCCCTGCACATTGCGTAATATCGTACGCTGCA4310.10336871693280314No Hit
GTCCACGAGGTCTCTAGTACAAACTAACGGGTTGCCGTACGCTGCA4250.10192970927248571No Hit
GTCCACGAGGTCTCTAGATGTACCCAATTCTTCGACGTACGCTGCA4230.10145004005237988No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA4230.10145004005237988No Hit
GTCCACGAGGTCTCTGAAGAGTTCAAGCCATCCCCGTACGCTGCAG4200.10073053622222117No Hit
GTCCACGAGGTCTCTACGAACGTGGCATGTTTGATCGTACGCTGCA4180.10025086700211534No Hit
GTCCACGAGGTCTCTACCCAGTAGTGAACAGGAGCCGTACGCTGCA4180.10025086700211534No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA4170.10001103239206242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC304.860043E-640.00000430
TCCGGAA600.040.00000415
TCGCCTA304.860043E-640.00000415
GTTTGTA304.860043E-640.00000433
AGCGTTT304.860043E-640.00000416
GTTTGAT304.860043E-640.00000429
CTATGTA1200.040.00000414
TTAGGTC304.860043E-640.00000415
TTCTAGT304.860043E-640.00000429
AACAATG304.860043E-640.00000424
TAATATG304.860043E-640.00000415
CGTGGGC304.860043E-640.00000428
CGGGTAG304.860043E-640.00000429
GAAATAA304.860043E-640.00000420
AACAACG304.860043E-640.00000424
TAATAAT304.860043E-640.00000425
CTGTCCG1200.040.00000431
AAATCGC304.860043E-640.00000425
CCGGGAG304.860043E-640.00000416
CGTATTG304.860043E-640.00000417