FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_40.3510000004f5de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_40.3510000004f5de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36758
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA8772.3858751836334946Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC5631.5316393710212741No Hit
GAGTAAACATAGTGCCATGCTCAGGAACAAAGAAACGCGGCACAGA3791.031068066815387No Hit
CTTCGGGATGAAAATGCTCACAATGACAAATCTGTCCACGGAGTGC3140.8542358126122205No Hit
TGGAGAAAGACGGAGAGCGCCAACGGCGTCCATCTCGAAGGAGTCG2380.647478100005441No Hit
AAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGTAGCCGACGT2310.6284346264758692No Hit
GGCTAATGATTCACACGCCGACTGCTATCAGTATTTTTGTGTGCCT2170.5903476794167256No Hit
AGCATTGGGATTATCATAAAACGCCTCTAATCGGTCGTCAGCCAAC2050.5577017247946026No Hit
TCGCCGCCAGTTAAATAGCTTGCAAAATACGTGGCCTTATGGTTAC2040.5549812285760923No Hit
GGTAAACGCGAACAATTCAGCGGCTTTAACCGGACGCTCGACGCCA1990.541378747483541No Hit
GCTGGCTGCTGAACGCCCTCTTAAGGATATTCGCGATGAGTATAAT1900.5168942815169487No Hit
GGTTAGTGCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAATCAG1840.5005713042058871No Hit
GCCTCAACGCAGCGACGAGCACGAGAGCGGTCAGTAGCAATCCAAA1670.4543228684912128No Hit
CGGCTGCGGACGACCAGGGCGAGCGCCAGAACGTTTTTTACCTTTA1520.41351542521355894No Hit
TCTGCCGACCACTCGCGATTCAATCATGACTTCGTGATAAAAGATT1490.40535393655802815No Hit
CCTGTGACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAA1420.3863104630284564No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA1270.34550301975080255No Hit
CGCGAGCAGTAGACTCCTTCTGTTGATAAGCAAGCATCTCATTTTG1110.30197508025463843No Hit
AGTAAGAGCTTCTCGAGCTGCGCAAGGATAGGTCGAATTTTCTCAT1050.2856521029435769No Hit
GGATAGGTCGAATTTTCTCATTTTCCGCCAGCAGTCCACTTCGATT930.25300614832145385No Hit
GTCCACGAGGTCTCTATGTGATTCCCACTCAGGCGCGTACGCTGCA770.20947820882528975No Hit
GTCCACGAGGTCTCTCATGTAGGCTAATCGGATACGTACGCTGCAG650.17683225420316667No Hit
GTCCACGAGGTCTCTACTACCAGTCGATATGAGGGCGTACGCTGCA640.1741117579846564No Hit
GTCCACGAGGTCTCTGGCCCGCACACAATTAGGAACGTACGCTGCA580.15778878067359486No Hit
GTCCACGAGGTCTCTTGGTAGACCCACAAACTACCGTACGCTGCAG560.15234778823657436No Hit
GTCCACGAGGTCTCTAAGCCGGTCGTAACTTGTACCGTACGCTGCA560.15234778823657436No Hit
GTCCACGAGGTCTCTCATCGATCACAGTTAGTCTGTACGCTGCAGG520.14146580336253334No Hit
GTCCACGAGGTCTCTATCTCATGGGCTCGTACGCTGCAGGTCGACA500.1360248109255128No Hit
GTCCACGAGGTCTCTAGATGACACAACCCGGTGCACGTACGCTGCA500.1360248109255128No Hit
GTCCACGAGGTCTCTTAGAGCCTCAGCATTCATCGCGTACGCTGCA490.13330431470700255No Hit
GTCCACGAGGTCTCTTTACGGAGGCTTGGCATATTCGTACGCTGCA470.12786332226998207No Hit
GTCCACGAGGTCTCTGAAGTGGTCGTCATACTCTCGTACGCTGCAG460.12514282605147178No Hit
GTCCACGAGGTCTCTTCGTATAGCAAACGGGCGACGTACGCTGCAG450.12242232983296153No Hit
GTCCACGAGGTCTCTGAAGCACGCGCAAGCATCTACGTACGCTGCA440.11970183361445129No Hit
GTCCACGAGGTCTCTGGCTGGCACAAGATACCACGTACGCTGCAGG430.116981337395941No Hit
GTCCACGAGGTCTCTCGATCCACAGTAGCTGAATCCGTACGCTGCA420.11426084117743077No Hit
GTCCACGAGGTCTCTGACTAGGACCGATTGCACTTCGTACGCTGCA410.11154034495892051No Hit
GTCCACGAGGTCTCTGTCCTGAGCACAAGAGCACACGTACGCTGCA410.11154034495892051No Hit
GTCCACGAGGTCTCTTATTGGGAGCCAGGTTTCATCGTACGCTGCA400.10881984874041026No Hit
GTCCACGAGGTCTCTTAAGATGTGTGAACTGTGTCCGTACGCTGCA400.10881984874041026No Hit
GTCCACGAGGTCTCTGGCTAGAACCAATTCCCAGACGTACGCTGCA390.10609935252189999No Hit
GTCCACGAGGTCTCTCCGGTTGCCAAAGCGAGAAACGTACGCTGCA390.10609935252189999No Hit
GTCCACGAGGTCTCTAGATGGCTGCTCATGCTCCTCGTACGCTGCA390.10609935252189999No Hit
GTCCACGAGGTCTCTAGTCGGATCACCCGCTGAATCGTACGCTGCA390.10609935252189999No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA390.10609935252189999No Hit
GTCCACGAGGTCTCTGTGGGCCTAACACAAGCACACGTACGCTGCA380.10337885630338974No Hit
GTCCACGAGGTCTCTGCACCCTTAACATTTGCTGCCGTACGCTGCA380.10337885630338974No Hit
GTCCACGAGGTCTCTGCCTATGTCACAATCTGTCACGTACGCTGCA380.10337885630338974No Hit
GTCCACGAGGTCTCTCAGACAGAGACAGGTGTACCCGTACGCTGCA380.10337885630338974No Hit
GTCCACGAGGTCTCTCTTGACAGGAGTGATAAACCGTACGCTGCAG380.10337885630338974No Hit
GTCCACGAGGTCTCTGGACACGCACACGTTAGCACGTACGCTGCAG370.1006583600848795No Hit
GTCCACGAGGTCTCTTGAGTACATAGACAGCACCCCGTACGCTGCA370.1006583600848795No Hit
GTCCACGAGGTCTCTTACGCGAGTGCATGTGCCATCGTACGCTGCA370.1006583600848795No Hit
GTCCACGAGGTCTCTATAACCACCGGGAAGTGTGACGTACGCTGCA370.1006583600848795No Hit
GTCCACGAGGTCTCTCTAGAACACGCATGTAGAGCCGTACGCTGCA370.1006583600848795No Hit
GTCCACGAGGTCTCTACAACGGGTTCTAACTCGCGCGTACGCTGCA370.1006583600848795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCGC555.456968E-1240.00000412
CTCTACT555.456968E-1240.00000412
TCTTATC1100.040.00000413
CTCTGGA507.2759576E-1140.012
CTCGAAG257.648187E-540.034
CGCGAAC257.648187E-540.07
CTCTGCT451.1532393E-940.012
CTCTGCC900.040.012
GAGGCGT257.648187E-540.032
AACGTTT200.001239976740.030
GAGGTCG700.040.07
CGTCAGC257.648187E-540.036
GGAGAGC257.648187E-540.012
GAGCGTA451.1532393E-940.033
TGGCATG200.001239976740.023
ACTGTCG900.040.027
GGAGAAA257.648187E-540.02
AACGCGA257.648187E-540.05
ACTGTAG352.9501098E-740.033
AACAATT257.648187E-540.011
Produced by FastQC (version 0.11.2)