000000000-AKRU9_l01n01_exp163gap1bff_4.3510000004f39d.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_4.3510000004f39d.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	188071
Sequences flagged as poor quality	0
Sequence length	46
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	22263	11.83755071223102	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	2094	1.11340929755252	No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	689	0.36635100573719503	No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA	578	0.3073307421133508	RNA PCR Primer, Index 41 (96% over 28bp)
AAAGTAAAAATGTCTACAGTAGAGTCAATAGCAAGGCCACGACGCA	529	0.2812767518649872	No Hit
GGCCACGTATTTTGCAAGCTATTTAACTGGCGGCGATTGCGTACCC	470	0.24990562074961054	No Hit
ACATTATTGCCCGGCGTACGGGGAAGGACGTCAATAGTCACACAGT	458	0.24352505170919495	No Hit
ACGTGACGATGAGGGACATAAAAAGTAAAAATGTCTACAGTAGAGT	400	0.2126856346805196	No Hit
AGTGCACCGCATGGAAATGAAGACGGCCATTAGCTGTACCATACTC	380	0.20205135294649362	No Hit
ATCACCTCACTTAAGTGGCTGGAGACAAATAATCTCTTTAATAACC	377	0.20045621068638972	No Hit
GCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAGAAGTCGTCA	326	0.17333879226462345	No Hit
CGATACCACTGACCCTCAGCAATCTTAAACTTCTTAGACGAATCAC	321	0.17068022183111697	No Hit
GGCTGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTACTGAGAAG	319	0.16961679365771437	No Hit
CGAGCATCATCTTGATTAAGCTCATTAGGGTTAGCCTCGGTACGGT	299	0.1589825119236884	No Hit
TGTCAATAGATGTGGTAGAAGTCGTCATTTGGCGAGAAAGCTCAGT	293	0.1557922274034806	No Hit
TAAGCAATGACGGCAGCAATAAACTCAACAGGAGCAGGAAAGCGAG	287	0.1526019428832728	No Hit
GAGGTTTTACCTCCAAATGAAGAAATAACATCATGGTAACGCTGCA	279	0.14834823018966242	No Hit
GCAGATGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTT	267	0.14196766114924683	No Hit
AAAAGCCTCCAAGATTTGGAGGCATGAAAACATACAATTGGGAGGG	265	0.14090423297584423	No Hit
TCATGGTGGCGAATAAGTACGCGTTCTTGCAAATCACCAGAAGGCG	244	0.12973823715511695	No Hit
ACGCAAGCCTCAACGCAGCGACGAGCACGAGAGCGGTCAGTAGCAA	229	0.12176252585459747	No Hit
AACGCCTCTAATCGGTCGTCAGCCAACGTGAGAGTGTCAAAAACGA	227	0.12069909768119486	No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA	211	0.11219167229397407	No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA	205	0.10900138777376628	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACC	30	4.8457987E-6	40.000004	21
TTAGGTA	30	4.8457987E-6	40.000004	15
CTATGGT	30	4.8457987E-6	40.000004	14
GGGGAAG	60	0.0	40.000004	20
TTGGCGT	30	4.8457987E-6	40.000004	32
TCTGAAA	30	4.8457987E-6	40.000004	13
CTCAAAT	30	4.8457987E-6	40.000004	22
CTCCGGA	30	4.8457987E-6	40.000004	14
GAATGTT	30	4.8457987E-6	40.000004	25
TGAAGCG	30	4.8457987E-6	40.000004	31
ACGCAAT	30	4.8457987E-6	40.000004	22
CTTTTCA	30	4.8457987E-6	40.000004	14
CTTCAGG	30	4.8457987E-6	40.000004	14
TAGGTAG	30	4.8457987E-6	40.000004	16
CTGAGGT	60	0.0	40.000004	14
GACGTCA	60	0.0	40.000004	27
ACATTCA	30	4.8457987E-6	40.000004	25
CTCCATA	30	4.8457987E-6	40.000004	14
TTCAACT	30	4.8457987E-6	40.000004	24
CTCCACT	30	4.8457987E-6	40.000004	14

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content