FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_39.3510000004f5c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_39.3510000004f5c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159664
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA24101.5094197815412367Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC18341.1486621906002605No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA6380.399589137188095No Hit
ATTTACGGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCC4880.30564184788054916No Hit
ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGG3890.2436366369375689No Hit
TAAAGGAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAG3600.22547349433811004No Hit
TTATTTTGACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATTGAG3580.2242208638140094No Hit
GTCCACGAGGTCTCTGCAATGCAAGCTGTGCCAACCGTACGCTGCA3120.19541036175969537No Hit
CAACCGTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCATCTGGC3070.19227878544944382No Hit
ATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAACACTGACGTT3060.19165247018739354No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA2920.18288405651868925No Hit
GTCCACGAGGTCTCTTATCGCTAGGCATGGTCCTGCGTACGCTGCA2890.18100511073253833No Hit
GTCCACGAGGTCTCTAGAGGTATAACACTCACCCCGTACGCTGCAG2710.16973143601563281No Hit
ATAGCAAAGCCTCTACGCGATTTCATAGTGGAGGCCTCCAGCAATC2700.16910512075358253No Hit
GTCCACGAGGTCTCTAGATGCTCATAACGCTGCTCCGTACGCTGCA2570.16096302234692855No Hit
GTCCACGAGGTCTCTTACTGCGGTACAGGCATTTGCGTACGCTGCA2500.1565788155125764No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG2440.15282092394027458No Hit
TGCTTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAATCAAAAA2380.14906303236797275No Hit
GTCCACGAGGTCTCTCCTGCTCGACAACTAGAAGACGTACGCTGCA2300.1440525102715703No Hit
TGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGA2290.14342619500951997No Hit
TTCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTAACCTTGC2230.13966830343721817No Hit
GTCCACGAGGTCTCTCCACGAGACGTTTCTAAATCCGTACGCTGCA2210.13841567291311754No Hit
GTCCACGAGGTCTCTTATGAGGGCTCGACCCATTCGTACGCTGCAG2040.12776831345826234No Hit
GTCCACGAGGTCTCTGAGTCGTCTCTCACTGCGTTCGTACGCTGCA2040.12776831345826234No Hit
GTCCACGAGGTCTCTATCTCATGGGCTCGTACGCTGCAGGTCGACA2020.12651568293416174No Hit
GTCCACGAGGTCTCTCCACTGTGTAAAGACTCGGACGTACGCTGCA2010.12588936767211142No Hit
GGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTAT2010.12588936767211142No Hit
GTCCACGAGGTCTCTGGATTATACCGACCACATTCCGTACGCTGCA1990.12463673714801081No Hit
GTCCACGAGGTCTCTCCGGTTTGAGAATATCTTCTCGTACGCTGCA1970.12338410662391022No Hit
GTCCACGAGGTCTCTGCCATGTATTAAGATAGCGCGTACGCTGCAG1960.12275779136185991No Hit
GTCCACGAGGTCTCTGAGATGTATCCCACCATATCCGTACGCTGCA1940.12150516083775928No Hit
GTCCACGAGGTCTCTTAAGCTAGTATAACCGCTGCCGTACGCTGCA1910.11962621505160838No Hit
GTCCACGAGGTCTCTTAACAGGCGATACACCTACGTACGCTGCAGG1900.11899989978955806No Hit
GTCCACGAGGTCTCTGGCCCGCACACAATTAGGAACGTACGCTGCA1900.11899989978955806No Hit
GTCCACGAGGTCTCTAACATACGAGGCGGTGCAATCGTACGCTGCA1890.11837358452750776No Hit
GTCCACGAGGTCTCTCATCTCAGGCAACGTGATTACGTACGCTGCA1870.11712095400340716No Hit
GTCCACGAGGTCTCTAGAGTGTCCCAAGTTCCCTTCGTACGCTGCA1860.11649463874135685No Hit
GTCCACGAGGTCTCTATGTGATTCCCACTCAGGCGCGTACGCTGCA1830.11461569295520595No Hit
GTCCACGAGGTCTCTTGTGGAGCCCAACAAAGCCACGTACGCTGCA1830.11461569295520595No Hit
GTCCACGAGGTCTCTAGACGGACTCGCATTCACTGCGTACGCTGCA1820.11398937769315563No Hit
GTCCACGAGGTCTCTCACTGTGAGTAATGATGACGCGTACGCTGCA1790.1121104319070047No Hit
GTCCACGAGGTCTCTGAGCTGAGTGAATCTTTCTCCGTACGCTGCA1790.1121104319070047No Hit
GTCCACGAGGTCTCTCGACTAGCATGACATTTCAGCGTACGCTGCA1780.11148411664495442No Hit
GTCCACGAGGTCTCTAATGATGTTGAATGTGCCCGCGTACGCTGCA1770.1108578013829041No Hit
GTCCACGAGGTCTCTCCCGTGAGGCTAGAGATTATCGTACGCTGCA1760.11023148612085379No Hit
GGGGTTGACCAAGCGAAGCGCGGTAGGTTTTCTGCTTAGGAGTTTA1760.11023148612085379No Hit
GTCCACGAGGTCTCTGCGCACTACAAGGCTGACGTACGCTGCAGGT1740.10897885559675317No Hit
GTCCACGAGGTCTCTTGAAACAACACACGCCGGTGCGTACGCTGCA1740.10897885559675317No Hit
GTCCACGAGGTCTCTTAACGGAGGCGAACTTAGCCGTACGCTGCAG1730.10835254033470289No Hit
GTCCACGAGGTCTCTCAAGCCTGGGTCACATGATTCGTACGCTGCA1730.10835254033470289No Hit
GTCCACGAGGTCTCTGTAGTAGACCCATGACACTCCGTACGCTGCA1720.10772622507265257No Hit
GTCCACGAGGTCTCTGATACTGTCACACCGTTGCGCGTACGCTGCA1710.10709990981060226No Hit
GTCCACGAGGTCTCTTCAGAGATTACATGTCCCTGCGTACGCTGCA1700.10647359454855196No Hit
ACGCCCTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAG1690.10584727928650164No Hit
GTCCACGAGGTCTCTAGCGAGCACACATTCATTACCGTACGCTGCA1680.10522096402445136No Hit
GTCCACGAGGTCTCTCGATCCACAGTAGCTGAATCCGTACGCTGCA1680.10522096402445136No Hit
GTCCACGAGGTCTCTTTGTACCGGCTTCCAGGCTCCGTACGCTGCA1680.10522096402445136No Hit
GTCCACGAGGTCTCTTACGCGAGTGCATGTGCCATCGTACGCTGCA1680.10522096402445136No Hit
GTCCACGAGGTCTCTTTAGGCTATGCACCGCTCTGCGTACGCTGCA1680.10522096402445136No Hit
GTCCACGAGGTCTCTGTGCATATAAGACACCGTACCGTACGCTGCA1670.10459464876240104No Hit
GTCCACGAGGTCTCTTGAGGAACACGGTGCAATACCGTACGCTGCA1670.10459464876240104No Hit
GTCCACGAGGTCTCTGATAGATAAGCACGCAGCCCCGTACGCTGCA1660.10396833350035074No Hit
GTCCACGAGGTCTCTCGGAGCTACTGATGTCTATTCGTACGCTGCA1650.10334201823830043No Hit
GTCCACGAGGTCTCTGAGATGTGGATCTCACCCATCGTACGCTGCA1650.10334201823830043No Hit
GTCCACGAGGTCTCTGGCCTGAGAGTCATTTGAATTGTACGCTGCA1640.10271570297625011No Hit
GTCCACGAGGTCTCTCGCCACACTTGTTAAATACCGTACGCTGCAG1630.10208938771419983No Hit
GTCCACGAGGTCTCTCCTAGAGGCTAATTTGCATCCGTACGCTGCA1630.10208938771419983No Hit
GTCCACGAGGTCTCTGTAATGTCCCTTAACCTACGCGTACGCTGCA1620.10146307245214951No Hit
GTCCACGAGGTCTCTCAGACAGAGACAGGTGTACCCGTACGCTGCA1620.10146307245214951No Hit
GTCCACGAGGTCTCTACAGCGTTGAAAGTAGCTCACGTACGCTGCA1610.10083675719009921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCAT401.9104846E-840.00000415
TCAAAAA200.001252523940.00000440
CGGACAA200.001252523940.00000422
TCCGGAA304.8411894E-640.00000415
CCTTCGC200.001252523940.00000428
CTTGTGG304.8411894E-640.00000414
ACAACTA200.001252523940.00000424
TATCATG200.001252523940.00000415
ATTTACC200.001252523940.00000421
ATCCTGT200.001252523940.00000428
TGTTTGG200.001252523940.00000435
GTTTGGA200.001252523940.00000429
GTTTGCT200.001252523940.0000047
GTGCAGG200.001252523940.00000431
AACAATC200.001252523940.00000424
GAGTGTG200.001252523940.00000428
CATGGGC401.9104846E-840.00000420
CAGGTAC200.001252523940.00000434
GTGCAAC304.8411894E-640.00000422
AGCAATT200.001252523940.00000423
Produced by FastQC (version 0.11.2)