000000000-AKRU9_l01n01_exp163gap1bff_38.3510000004f5b4.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_38.3510000004f5b4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	267359
Sequences flagged as poor quality	0
Sequence length	46
%GC	56

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	2771	1.0364341578177656	No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	2767	1.0349380421081766	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	1619	0.6055528334561395	No Hit
GTCCACGAGGTCTCTGGCCCGCACACAATTAGGAACGTACGCTGCA	460	0.17205330660273266	No Hit
GGCTGGAGACAAATAATCTCTTTAATAACCTGATTCAGCGAAACCA	459	0.17167927767533542	No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA	441	0.164946756982185	No Hit
GTCCACGAGGTCTCTATGACGTGTCGATCACTCCTCGTACGCTGCA	436	0.1630766123451988	No Hit
GTCCACGAGGTCTCTGCAGAAGTAAGCCCTCGACGTACGCTGCAGG	422	0.15784020736163734	No Hit
GTCCACGAGGTCTCTAGAGGTATAACACTCACCCCGTACGCTGCAG	381	0.1425050213383503	No Hit
CAAAGCACCTTTAGCGTTAAGGTACTGAATCTCTTTAGTCGCAGTA	380	0.14213099241095306	No Hit
GTCCACGAGGTCTCTCGACTAGCATGACATTTCAGCGTACGCTGCA	378	0.14138293455615858	No Hit
GTCCACGAGGTCTCTACAGCAGGGCAAGTCATGTACGTACGCTGCA	370	0.1383907031369806	No Hit
GTCCACGAGGTCTCTAGACGGACTCGCATTCACTGCGTACGCTGCA	352	0.1316581824438302	No Hit
GTCCACGAGGTCTCTGCGGCTGCAATACAGTTCTACGTACGCTGCA	349	0.13053609566163846	No Hit
GTCCACGAGGTCTCTCCTGCGCGGCAAGAACATAACGTACGCTGCA	344	0.12866595102465225	No Hit
GTCCACGAGGTCTCTCCTAGAGGCTAATTTGCATCCGTACGCTGCA	335	0.12529969067807703	No Hit
GTCCACGAGGTCTCTCAGACGAGCTAATTCTATCCCGTACGCTGCA	327	0.12230745925889909	No Hit
GTCCACGAGGTCTCTAACCATTAAGCAGACGTGGCCGTACGCTGCA	327	0.12230745925889909	No Hit
GTCCACGAGGTCTCTCCCTGCACATTGCGTAATATCGTACGCTGCA	327	0.12230745925889909	No Hit
GTCCACGAGGTCTCTATCATGTGCCGATGCTCGTTTCGTACGCTGC	327	0.12230745925889909	No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA	324	0.12118537247670735	No Hit
GTCCACGAGGTCTCTCGCACGCTCTGCATTGATATCGTACGCTGCA	320	0.11968925676711836	No Hit
GTCCACGAGGTCTCTTAAGCTAGTATAACCGCTGCCGTACGCTGCA	317	0.11856716998492664	No Hit
GTCCACGAGGTCTCTATCTCATGGGCTCGTACGCTGCAGGTCGACA	315	0.11781911213013214	No Hit
GTCCACGAGGTCTCTTGCATGGCCTTACAGGTCTGCGTACGCTGCA	315	0.11781911213013214	No Hit
GTCCACGAGGTCTCTGGTGCTCAGTCATCTTCACTCGTACGCTGCA	313	0.11707105427533765	No Hit
GTCCACGAGGTCTCTAATCTCTATGCAGTGAGGTGCGTACGCTGCA	312	0.11669702534794042	No Hit
GTCCACGAGGTCTCTGAGATGTGGATCTCACCCATCGTACGCTGCA	304	0.11370479392876244	No Hit
GTCCACGAGGTCTCTGGCCTGAGAGTCATTTGAATTGTACGCTGCA	304	0.11370479392876244	No Hit
GTCCACGAGGTCTCTCATCAGGAAGCAAGCCTTTGACGTACGCTGC	297	0.11108659143698174	No Hit
GTCCACGAGGTCTCTTAACGGAGGCGAACTTAGCCGTACGCTGCAG	294	0.10996450465479	No Hit
GAGGTGATTTATGTTTGGTGCTATTGCTGGCGGTATTGCTTCTGCT	292	0.10921644679999551	No Hit
GTCCACGAGGTCTCTCGATCCACAGTAGCTGAATCCGTACGCTGCA	292	0.10921644679999551	No Hit
TGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGG	290	0.10846838894520103	No Hit
GTCCACGAGGTCTCTCCGGAAGGTGTTGTAACTATCGTACGCTGCA	288	0.10772033109040655	No Hit
ACGCGTTCTTGCAAATCACCAGAAGGCGGTTCCTGAATGAATGGGA	287	0.10734630216300929	No Hit
GTCCACGAGGTCTCTAGTCGGATCACCCGCTGAATCGTACGCTGCA	284	0.10622421538081755	No Hit
GTCCACGAGGTCTCTTTGCCCAGAGAGGAAACGCCACGTACGCTGC	283	0.1058501864534203	No Hit
GTCCACGAGGTCTCTAGCGAGCACACATTCATTACCGTACGCTGCA	283	0.1058501864534203	No Hit
GTCCACGAGGTCTCTGATAGATCCATCACGCTCCGTACGCTGCAGG	282	0.10547615752602307	No Hit
GTCCACGAGGTCTCTCCAGGTAGTGAACTGCTATCCGTACGCTGCA	279	0.10435407074383134	No Hit
GTCCACGAGGTCTCTAGATCATTTGAATGCCACTCTGTACGCTGCA	279	0.10435407074383134	No Hit
GTCCACGAGGTCTCTAGATGCTCATAACGCTGCTCCGTACGCTGCA	277	0.10360601288903683	No Hit
GTCCACGAGGTCTCTACAGCGTTGAAAGTAGCTCACGTACGCTGCA	276	0.1032319839616396	No Hit
GTCCACGAGGTCTCTCTGAAGGAGCAATTTCGAGACGTACGCTGCA	275	0.10285795503424235	No Hit
GTCCACGAGGTCTCTGCTACACCAACATAGGTTCACGTACGCTGCA	275	0.10285795503424235	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAA	30	4.853491E-6	40.000004	15
TGATCAG	30	4.853491E-6	40.000004	15
CCTTCGG	30	4.853491E-6	40.000004	28
TCGCCTA	30	4.853491E-6	40.000004	15
TCTGCCT	290	0.0	40.000004	13
GTTTGTC	30	4.853491E-6	40.000004	30
TTGATGC	30	4.853491E-6	40.000004	30
ACAACGC	30	4.853491E-6	40.000004	25
GTTGAAA	30	4.853491E-6	40.000004	21
TTAGGTT	30	4.853491E-6	40.000004	28
AACCGCC	30	4.853491E-6	40.000004	26
GTCGCCG	30	4.853491E-6	40.000004	31
GTACCAA	30	4.853491E-6	40.000004	19
CATATTA	30	4.853491E-6	40.000004	26
GTCGAGG	30	4.853491E-6	40.000004	29
TGCTTGC	30	4.853491E-6	40.000004	15
GGGCTCT	30	4.853491E-6	40.000004	17
AGTCAAT	30	4.853491E-6	40.000004	22
TGCTTCA	30	4.853491E-6	40.000004	19
CCGATTC	30	4.853491E-6	40.000004	29

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content