FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_37.3510000004f5a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_37.3510000004f5a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202695
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA150577.428402279286613Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC34201.6872641160364095No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA8600.42428278941266434No Hit
AAACATACGAAGGCGCATAACGATACCACTGACCCTCAGCAATCTT4740.2338488862576778No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA3930.19388736771997336RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTCGATCCACAGTAGCTGAATCCGTACGCTGCA3470.17119317200720294No Hit
GTCCACGAGGTCTCTTTACGGAGGCTTGGCATATTCGTACGCTGCA3450.17020646784577814No Hit
GAAGCGGAGCAGTCCAAATGTTTTTGAGATGGCAGCAACGGAAACC3340.16477959495794173No Hit
CTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACTTCC3170.15639260958583093No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA3110.15343249710155651No Hit
GTCCACGAGGTCTCTAGACGGACTCGCATTCACTGCGTACGCTGCA2980.14701892005229533No Hit
CAGGAAAGCGAGGGTATCCTACAAAGTCCAGCGTACCATAAACGCA2920.14405880756802092No Hit
GTCCACGAGGTCTCTGGCCCGCACACAATTAGGAACGTACGCTGCA2880.14208539924517133No Hit
TGGCATTAACACCATCCTTCATGAACTTAATCCACTGTTCACCATA2870.14159204716445892No Hit
GTCCACGAGGTCTCTTGGAGATAGACAGACCACCCCGTACGCTGCA2650.1307383013887861No Hit
TGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGT2620.1292582451466489No Hit
GTCCACGAGGTCTCTGAGATGTGGATCTCACCCATCGTACGCTGCA2590.1277781889045117No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA2540.1253114285009497No Hit
GTCCACGAGGTCTCTGCCTATGTCACAATCTGTCACGTACGCTGCA2460.12136461185525049No Hit
GTCCACGAGGTCTCTGATGCGGCACTATCGCTCATCGTACGCTGCA2370.1169244431288389No Hit
GTCCACGAGGTCTCTAACACTGGTAAACGGTGCGACGTACGCTGCA2290.11297762648313969No Hit
GTCCACGAGGTCTCTACTACTGGCTTAGATGTGGTCGTACGCTGCA2280.1124842744024273No Hit
GTCCACGAGGTCTCTTATTGGGAGCCAGGTTTCATCGTACGCTGCA2220.10952416191815288No Hit
GTCCACGAGGTCTCTATACGTTAGACAGGTCAGCCATACGCTGCAG2160.10656404943387848No Hit
GTCCACGAGGTCTCTAGATGTAGAGCATATACCGGCGTACGCTGCA2130.10508399319174129No Hit
GTCCACGAGGTCTCTGAGATCATGCTATTCAAGCCCGTACGCTGCA2120.10459064111102889No Hit
GTCCACGAGGTCTCTGGCTAATACTTCTGAACCCCCGTACGCTGCA2100.10360393694960408No Hit
GTCCACGAGGTCTCTGGGAAATATAAATCTGGCCCCGTACGCTGCA2040.10064382446532968No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA2040.10064382446532968No Hit
GTCCACGAGGTCTCTATCAGACTGCCTAATGGGCCCGTACGCTGCA2030.10015047238461727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTGC257.771918E-540.00000430
ATCCTGT257.771918E-540.00000428
AGCGTTT257.771918E-540.00000416
CTTCGCG257.771918E-540.00000414
ATCCTAC257.771918E-540.00000416
TTAGGTG257.771918E-540.00000426
AAACTCT257.771918E-540.00000425
ATGGGCT257.771918E-540.00000421
CGGAAGA257.771918E-540.00000423
AGCAAAT257.771918E-540.00000422
AGCAAAG257.771918E-540.00000418
GCCTTGC257.771918E-540.00000418
AAATCTA257.771918E-540.00000425
GCCTTAC257.771918E-540.00000422
GGTACCG257.771918E-540.00000431
ACAAAGT257.771918E-540.00000421
CAAACAA257.771918E-540.00000420
CGAACCA1000.040.00000424
TCACCTT257.771918E-540.00000416
ACTGCAA257.771918E-540.00000421
Produced by FastQC (version 0.11.2)