000000000-AKRU9_l01n01_exp163gap1bff_36.3510000004f59b.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_36.3510000004f59b.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	568955
Sequences flagged as poor quality	0
Sequence length	46
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	371769	65.34242602666292	Illumina PCR Primer Index 11 (96% over 28bp)
GCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	17977	3.1596523450888028	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA	9350	1.6433637106625303	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA	4454	0.7828387130792418	RNA PCR Primer, Index 41 (96% over 30bp)
GCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC	4084	0.7178072079514197	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	3912	0.6875763461082159	RNA PCR Primer, Index 41 (96% over 30bp)
GCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA	3811	0.6698245028165672	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA	2823	0.4961728080428153	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	2821	0.4958212863934758	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA	2601	0.45715390496612207	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGCAAAAAA	1916	0.3367577400673164	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	1379	0.2423741772196395	No Hit
GCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACC	1300	0.22848907207072616	RNA PCR Primer, Index 41 (96% over 30bp)
GCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT	1263	0.22198592155794394	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCGACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	1178	0.20704625146101188	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCGTGAAAAAAA	924	0.16240300199488536	RNA PCR Primer, Index 41 (96% over 28bp)
GCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG	899	0.15800898137814062	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	686	0.1205719257234755	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGCAAAACA	675	0.11863855665210782	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCGACATATCGTATGCCGTCTTCTGCTTGAAAAAAA	605	0.10633529892522256	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAC	579	0.10176551748380804	Illumina PCR Primer Index 11 (96% over 28bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTTGA	20	0.0012552341	40.000004	32
CGTCATT	20	0.0012552341	40.000004	24
TTGTTTC	20	0.0012552341	40.000004	8
ACACGTA	40	1.9254003E-8	40.000004	33
TTCACTT	20	0.0012552341	40.000004	15
TTTTGAT	20	0.0012552341	40.000004	33
GGTTGTT	20	0.0012552341	40.000004	6
GAGAAAG	20	0.0012552341	40.000004	22
GTCATTG	20	0.0012552341	40.000004	25
ACTAAAT	20	0.0012552341	40.000004	1
CTATATA	20	0.0012552341	40.000004	14
TTGCAAT	20	0.0012552341	40.000004	37
CACACTC	40	1.9254003E-8	40.000004	16
ACTCGAT	20	0.0012552341	40.000004	26
AATCTCA	20	0.0012552341	40.000004	26
CCGTGAG	20	0.0012552341	40.000004	17
CTATAGT	20	0.0012552341	40.000004	14
TCAAGCT	20	0.0012552341	40.000004	28
TAAATCA	20	0.0012552341	40.000004	24
CCCAGAT	20	0.0012552341	40.000004	17

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content