FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_35.3510000004f58e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_35.3510000004f58e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561
Sequences flagged as poor quality0
Sequence length46
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATAATCTCTTTAATAACCTGATTCAGCGAAACCAATCCGCGGC37423.959000640614992No Hit
GCGCCAACGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAG30419.47469570787956No Hit
GGAGTGGCATTAACACCATCCTTCATGAACTTAATCCACTGTTCAC25416.27162075592569No Hit
AACATACGAAGGCGCATAACGATACCACTGACCCTCAGCAATCTTA21914.029468289557975No Hit
AAGACGGAGAGCGCCAACGGCGTCCATCTCGAAGGAGTCGCCAGCG16810.762331838565023No Hit
AGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTAGATA1258.00768737988469No Hit
AGTGACTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTAGATA30.19218449711723257No Hit
GGAGTGGCATTAACACCATCCTTCATGAACTTAATCCACCGTTCAC20.12812299807815503No Hit
CAACTAATCTCTTTAATAACCTGATTCAGCGAAACCAATCCGCGGC20.12812299807815503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCATG209.0670824E-440.021
ACTGTTC209.0670824E-440.038
TGGTTTA209.0670824E-440.036
TGGCATT209.0670824E-440.05
CGTCAAG209.0670824E-440.026
ACCGGAG254.7554953E-540.040
AATCTTA302.4167039E-640.040
TCCTTCA209.0670824E-440.019
TTCATGA209.0670824E-440.022
AAACCAA405.627953E-940.032
GGCATTA209.0670824E-440.06
ATCTCTT405.627953E-940.07
TGGCACG209.0670824E-440.017
AATCCGC405.627953E-940.037
CCAATCC405.627953E-940.035
CATGAAC209.0670824E-440.024
AACCGGA254.7554953E-540.039
AATCTCT405.627953E-940.06
AATCCAC209.0670824E-440.033
GGCACGT209.0670824E-440.018