FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_34.3510000004f571.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_34.3510000004f571.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357614
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC43071.2043711935215065No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA26500.7410224431929399Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA18240.5100471458052538No Hit
GTCCACGAGGTCTCTGTTGCACATAAACAGCTCCACGTACGCTGCA5260.1470859641960326No Hit
GTCCACGAGGTCTCTAGATGTACCCAATTCTTCGACGTACGCTGCA4890.13673961310239532No Hit
GTCCACGAGGTCTCTCAGACGAGCTAATTCTATCCCGTACGCTGCA4710.1317062531108961No Hit
TCAATAGCAAGGCCACGACGCAATGGAGAAAGACGGAGAGCGCCAA4430.12387658201300844No Hit
GTCCACGAGGTCTCTATGACGTGTCGATCACTCCTCGTACGCTGCA4390.12275805757045305No Hit
GTCCACGAGGTCTCTGCAGTATTCATAACTCTCCGTACGCTGCAGG4020.11241170647681578No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA3830.10709871537467773No Hit
GTCCACGAGGTCTCTCAGACACCTGAATTTGTAGCCGTACGCTGCA3700.10346351093637274No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG3660.10234498649381736No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA3630.10150609316190082No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT200.001254609340.015
TGATCGC200.001254609340.015
GCGAGAT200.001254609340.024
GCGAGAG257.783873E-540.025
CGGACAA507.6397555E-1140.022
TCCGGCT304.8581005E-640.015
CCCTGAT304.8581005E-640.017
TCCGGAA401.9219442E-840.015
CCTTCGT1250.040.032
CCTTCGC257.783873E-540.028
ATTTATT353.048499E-740.027
GTGTTTA257.783873E-540.028
CTTGTGG600.040.014
TTGATTA200.001254609340.017
GTTTGTT353.048499E-740.024
ATTTAGG257.783873E-540.018
CTTAAGT200.001254609340.014
GTTGAGG257.783873E-540.025
GTGTTGC200.001254609340.030
CTTAAGA353.048499E-740.014
Produced by FastQC (version 0.11.2)