FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_33.3510000004f564.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_33.3510000004f564.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCAATCCGCGGCATTTAGTAGCGGTAAAGTTAGACCAAACCATG76527.28245363766049No Hit
ACTGCGATGGGCATACTGTAACCATAAGGCCACGTATTTTGCAAGC45516.22681883024251No Hit
CGAGCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTCGTCA33611.982881597717546No Hit
ACCAAACAGGCAAAAAATTTAGGGTCGGCATCAAAAGCAATATCAG2759.807417974322396No Hit
TAACCGGAGTAGTTGAAATGGTAATAAGACGACCAATCTGACCAGC2659.450784593437946No Hit
CAGCTTGGTTTTTAGTGAGTTGTTCCATTCTTTAGCTCCTAGACCT2448.7018544935806No Hit
GGGTATCCTACAAAGTCCAGCGTACCATAAACGCAAGCCTCAACGC2177.738944365192582No Hit
TAAAACTCTGCAGGTTGGATACGCCAATCATTTTTATCGAAGCGCG1174.172610556348074No Hit
AGCGCGGTAGGTTTTCTGCTTAGGAGTTTAATCATGTTTCAGACTT401.4265335235378032No Hit
ACCCAATCCGCGGCATTTAGTAGCGGTAAAGTTAGACCAAACCATG30.10699001426533523No Hit
AACCACTCCGCGGCATTTAGTAGCGGTAAAGTTAGACCAAACCATG30.10699001426533523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACC900.040.032
GATGGGC351.8523497E-740.06
TAGCGGT900.040.021
AAACTTT401.0086296E-840.029
ACTGCGA351.8523497E-740.01
TTTTGCA351.8523497E-740.037
ATGGGCA351.8523497E-740.07
ACTCGTC351.8523497E-740.039
ACTGTAA351.8523497E-740.015
TTGTTAC401.0086296E-840.034
ACCGGAG455.4023985E-1040.03
GAGCGGT401.0086296E-840.010
AAACCAT900.040.039
GTTGAAA455.4023985E-1040.012
CGGAGTA455.4023985E-1040.05
GGCATTT900.040.012
CCGGAGT455.4023985E-1040.04
GGGTCGG351.8523497E-740.022
AATCCGC900.040.05
TTGCAAG351.8523497E-740.039
Produced by FastQC (version 0.11.2)