FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_32.3510000004f558.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_32.3510000004f558.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103761
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA1125710.848970229662397Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA69086.657607386204837Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC40883.9398232476556703Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA23722.2860226867512843Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT16591.5988666261890307Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACC12541.2085465637378205Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG10381.000375863763842Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATACA8720.8403928258208768Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTT8700.8384653193396362Illumina PCR Primer Index 11 (96% over 27bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA8530.8220815142490916Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTGCA7650.7372712290745077Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTACA6830.6582434633436455Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA6560.6322221258468982Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTCGCTCTTCCGATCTCTGCAGGTCGACATCTC6230.6004182689064292No Hit
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAACA5510.5310280355817696Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTTCC5140.49536916567881956Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCACGCTCTTCCGATCTCTGCAGGTCGACATCTC4870.4693478281820722No Hit
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAA4840.46645656846021144Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC4700.4529640230915276No Hit
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATACC4680.4510365166102871Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAC4650.4481452568884263Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATAAA4540.4375439712416033Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTTCA4510.4346527115197425Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA4410.42501517911353975Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA4260.41055888050423567Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATGCA4180.4028488545792735Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTACC4100.3951388286543113Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAAAA4040.3893563092105897Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATC3980.38357378976686807Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAC3950.38068253004500724Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATCC3760.3623712184732221Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGCA3680.35466119254825995Illumina PCR Primer Index 11 (96% over 28bp)
ACGTACCTTCAAGAAGTCCTTTACCAGCTTTAGCCATAGCACCAGA3480.3353861277358545No Hit
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA3370.3247848420890315Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATCA3310.3190023226453099Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAT3250.31321980320158826Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAACC3250.31321980320158826Illumina PCR Primer Index 11 (96% over 28bp)
ACAACTGAACGGACTGGAAACACTGGTCATAATCATGGTGGCGAAT3070.2958722448704234No Hit
TGAAGTAATCACGTTCTTGGTCAGTATGCAAATTAGCATAAGCAGC2990.28816221894546123No Hit
TACGAAAAGACAGAATCTCTTCCAAGAGCTTGATGCGGTTATCCAT2980.2871984657048409No Hit
TTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCT2930.2823796995017396No Hit
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATT2710.2611771282080936Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCTCTTCCGATCTCTGCAGGTCGACATCTCG2700.2602133749674733No Hit
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAGCA2600.2505758425612706Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATAAC2580.24864833608003006Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATTCC2460.2370832971925868Illumina PCR Primer Index 11 (96% over 28bp)
GAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTTCGGATATT2350.2264820115457638No Hit
CGTAATTTTTGACGCACGTTTTCTTCTGCGTCAGTAAGAACGTCAG2350.2264820115457638No Hit
GTCCACGAGGTCTCGCTCTTCCGATCTCTGCAGGTCGACATCTCGT2330.22455450506452326No Hit
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAG2230.21491697265832058Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATTCA2210.21298946617708003Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGACGCTCTTCCGATCTCTGCAGGTCGACATCTCGTATGCC2180.2100982064552192No Hit
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACCA2170.20913445321459895Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAC2170.20913445321459895Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATACAG2150.20720694673335838Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAAAC2000.19275064812405432Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA1950.18793188192095298RNA PCR Primer, Index 41 (96% over 30bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACT1920.18504062219909215Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACATCA1910.18407686895847186Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACATCC1870.18022185599599078Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA1840.17733059627412998RNA PCR Primer, Index 41 (96% over 28bp)
AGCTGCTTATGCTAATTTGCATACTGACCAAGAACGTGATTACTTC1820.17540308979288943No Hit
GTCCACGAGGTCTCTTCCGATCTCTGCAGGTCGACATCTCGTATGC1790.17251183007102863No Hit
GTCCACGAGGTCTCTCTTCCGATCTCTGCAGGTCGACATCTCGTAT1710.16480180414606643No Hit
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTGCC1660.1599830379429651Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATACAA1570.15130925877738263Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGCAG1490.14359923285242046Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAAAA1480.1426354796118002Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCGTATGCCGTCTTCTGCTTGACGCTGCAGGTCG1470.14167172637117992TruSeq Adapter, Index 21 (96% over 26bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATACAT1400.13492545368683803Illumina PCR Primer Index 11 (96% over 28bp)
ATTAAAATTGTTGACCACCTACATACCAAAGACGAGCGCCTTTACG1400.13492545368683803No Hit
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACCC1380.1329979472055975Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCGTATGCCGTCTTCTGCTTTACGCTGCAGGTCG1380.1329979472055975TruSeq Adapter, Index 21 (96% over 25bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGC1370.1320341939649772Illumina PCR Primer Index 11 (96% over 28bp)
AAGGATATTTCTAATGTCGTCACTGATGCTGCTTCTGGTGTGGTTG1370.1320341939649772No Hit
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACACA1320.12721542776187583Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTCTCTTCCGATCTCTGCAGGTCGACATCTCGT1280.12336041479939477No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA1270.1223966615587745No Hit
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAT1240.11950540183691367Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATGAA1220.11757789535567313Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTACGCTCTTCCGATCTCTGCAGGTCGACATCT1210.11661414211505286No Hit
GTCCACGAGGTCTACGCTCTTCCGATCTCTGCAGGTCGACATCTCG1200.11565038887443259No Hit
GGTCGACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA1130.1089041161900907RNA PCR Primer, Index 41 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATTC1110.10697660970885015Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCGTATACGCTGCAGGTCGACATCTCGTATGCCG1100.10601285646822987No Hit
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAT1100.10601285646822987Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCAGGTCGACATCTCGTATGCCGTCTTCTGC1080.10408534998698933RNA PCR Primer, Index 41 (96% over 27bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTAAA1080.10408534998698933Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGAAA1070.10312159674636906Illumina PCR Primer Index 11 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGC200.001250496740.022
CAGCTCA200.001250496740.021
CTCTGGT451.198714E-940.012
CGTTCAT200.001250496740.022
TCCTTTA700.040.017
TTTTGCT200.001250496740.023
TTTTGAC257.74567E-540.07
TTGCATA200.001250496740.017
CTTCAAG700.040.07
ACTGTCG700.040.027
TGGTCAG257.74567E-540.018
ACACGCT200.001250496740.018
CAAGAAG700.040.010
CAAGAAC200.001250496740.029
GGTTGGA200.001250496740.01
TGCATAC200.001250496740.018
AGCTTTA700.040.026
CCAATCA200.001250496740.012
CTTTATG257.74567E-540.014
TCAAGAA700.040.09
Produced by FastQC (version 0.11.2)