FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_31.3510000004f54b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_31.3510000004f54b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4435
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAATTAGGGTCAACGCTACCTGTAGGAAGTGTCCGCATAAAGT60213.573844419391207No Hit
GCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTCGTCAGAA47610.732807215332581No Hit
TGAGGGACATAAAAAGTAAAAATGTCTACAGTAGAGTCAATAGCAA4279.627959413754228No Hit
GTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTG3427.711386696730553No Hit
ATCACCAGAACGGAAAACATCCTTCATAGAAATTTCACGCGGCGGC3317.463359639233371No Hit
TGTAGCTTTAGGTGTCTGTAAAACAGGTGCCGAAGAAGCTGGAGTA2856.426155580608793No Hit
CATTAGAAATATCCTTTGCAGTAGCGCCAATATGAGAAGAGCCATA2806.313416009019165No Hit
CAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAATGATTGGCGT2315.208568207440812No Hit
AAAATGGTTTTTAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGC2044.599774520856821No Hit
ACGTGCCAAGCATATTAAGCCACTTCTCCTCATCCAACGCGTCAGT1954.3968432919954905No Hit
CTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGC1814.081172491544533No Hit
CCGATTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGTGACTAT1753.9458850056369785No Hit
CCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCA1633.675310033821871No Hit
GCGCCTTTACGCTTGCCTTTAGTACCTCGCAACGGCTGCGGACGAC1172.6381059751972944No Hit
CCAGGGCGAGCGCCAGAACGTTTTTTACCTTTAGACATTACATCAC1092.45772266065389No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA110.2480270574971815Illumina PCR Primer Index 11 (96% over 28bp)
TGAGTGACATAAAAAGTAAAAATGTCTACAGTAGAGTCAATAGCAA50.11273957158962795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGG401.2883902E-840.00000422
CTTCATA200.001124374340.00000422
ATGGGTC401.2883902E-840.00000424
CTACAGT401.2883902E-840.00000426
GATGGGA200.001124374340.00000426
TGGTTTT200.001124374340.0000045
CAAGATG200.001124374340.00000423
ACAGTAG401.2883902E-840.00000428
GTAGAGT401.2883902E-840.00000431
TTTTGCC200.001124374340.00000438
ATGGGAA200.001124374340.00000427
GGGTCTG401.2883902E-840.00000426
TTCACGC200.001124374340.00000434
GAGAACG200.001124374340.00000414
TCCTTCA200.001124374340.00000420
CAGTTTT200.001124374340.00000435
AAGTAAA401.2883902E-840.00000414
TTCATAG200.001124374340.00000423
CCAATCT200.001124374340.0000041
AATCTGA200.001124374340.0000043
Produced by FastQC (version 0.11.2)