FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_30.3510000004f53e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_30.3510000004f53e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189782
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC20301.0696483333508973No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA19391.0216985804765468Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA7120.375167297214699No Hit
GCGGCGGCAAGTTGCCATACAAAACAGGGTCGCCAGCAATATCGGT3950.20813354269635687No Hit
GTCCACGAGGTCTCTGGCCCGCACACAATTAGGAACGTACGCTGCA3340.1759914006597043No Hit
GTCCACGAGGTCTCTAGATGGCTGCTCATGCTCCTCGTACGCTGCA3190.1680875952408553No Hit
GTCCACGAGGTCTCTCCTAGAGGCTAATTTGCATCCGTACGCTGCA2950.1554415065706969No Hit
CATAAACGTGACGATGAGGGACATAAAAAGTAAAAATGTCTACAGT2930.1543876658481837No Hit
GTCCACGAGGTCTCTTAACGGAGGCGAACTTAGCCGTACGCTGCAG2830.1491184622356177No Hit
GTCCACGAGGTCTCTAGAGGTATAACACTCACCCCGTACGCTGCAG2800.14753770115184792No Hit
GTCCACGAGGTCTCTCCTGTAGTACGAGTATGAGTCGTACGCTGCA2710.1427954179005385No Hit
GTCCACGAGGTCTCTCGTCTGACATAAGAGCAACCGTACGCTGCAG2690.14174157717802532No Hit
GTCCACGAGGTCTCTCCCAGCGAGCTAAAGATTCACGTACGCTGCA2690.14174157717802532No Hit
GTCCACGAGGTCTCTGGACTATACTCAATTCGACCCGTACGCTGCA2670.1406877364555121No Hit
GTCCACGAGGTCTCTATTGCCGCGTGCATGGCTATCGTACGCTGCA2640.1391069753717423No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA2600.1369992939267159No Hit
GTCCACGAGGTCTCTACAGCGTTGAAAGTAGCTCACGTACGCTGCA2560.13489161248168952No Hit
GTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAA2480.1306762495916367No Hit
GTCCACGAGGTCTCTCGACTAGCATGACATTTCAGCGTACGCTGCA2480.1306762495916367No Hit
GTCCACGAGGTCTCTTAAGCTAGTATAACCGCTGCCGTACGCTGCA2470.13014932923038014No Hit
GTCCACGAGGTCTCTCCATCTCAGTGGGTGCAATGCGTACGCTGCA2460.12962240886912352No Hit
GTCCACGAGGTCTCTGAGTCATGCACATTTCGGTCTGTACGCTGCA2440.1285685681466103No Hit
GTCCACGAGGTCTCTATGCTACGGCCACTAGGTTGCGTACGCTGCA2430.12804164778535373No Hit
GTCCACGAGGTCTCTGGTCCCGGCCCATAAAGAACGTACGCTGCAG2400.12646088670158392No Hit
GTCCACGAGGTCTCTATCATGTGCCGATGCTCGTTTCGTACGCTGC2380.1254070459790707No Hit
GTCCACGAGGTCTCTGTAGTAGACCCATGACACTCCGTACGCTGCA2380.1254070459790707No Hit
GTCCACGAGGTCTCTTAACAGGCGATACACCTACGTACGCTGCAGG2330.12277244417278772No Hit
GTCCACGAGGTCTCTGCGCTTATTAAATGTGCGGACGTACGCTGCA2330.12277244417278772No Hit
GTCCACGAGGTCTCTGCACTCGACTCATTTACATGCGTACGCTGCA2320.12224552381153111No Hit
GTCCACGAGGTCTCTATATGACCTGAACCGACGGCCGTACGCTGCA2320.12224552381153111No Hit
GTCCACGAGGTCTCTACTACTGGCTTAGATGTGGTCGTACGCTGCA2300.12119168308901793No Hit
GTCCACGAGGTCTCTTGCATGGCCTTACAGGTCTGCGTACGCTGCA2250.11855708128273493No Hit
GTCCACGAGGTCTCTCATCCGGTAACAGTATCTGCTGTACGCTGCA2220.11697632019896512No Hit
GCCAGAATACGAAAGACCAGGTATATGCACAAAATGAGATGCTTGC2200.11592247947645193No Hit
GTCCACGAGGTCTCTGCAGAAGTAAGCCCTCGACGTACGCTGCAGG2170.11434171839268213No Hit
GTCCACGAGGTCTCTAAGGCAGACTACTCGATCCGCGTACGCTGCA2160.11381479803142552No Hit
GTCCACGAGGTCTCTCATCAGTCTGGATAAGCTAGCGTACGCTGCA2110.11118019622514254No Hit
GTCCACGAGGTCTCTAGTACACTCCCATATTGTGCCGTACGCTGCA2080.10959943514137274No Hit
GTCCACGAGGTCTCTCCCTTTCTGGGAGCGTAGTTCGTACGCTGCA2070.10907251478011613No Hit
GTCCACGAGGTCTCTACAGCAGGGCAAGTCATGTACGTACGCTGCA2070.10907251478011613No Hit
GTCCACGAGGTCTCTTAGGTAATGACACTGAAGGCTACGCTGCAGG2050.10801867405760295No Hit
GTCCACGAGGTCTCTCCCGAACGTCGAATTGTTTACGTACGCTGCA2040.10749175369634634No Hit
GTCCACGAGGTCTCTGATAGATCCATCACGCTCCGTACGCTGCAGG2030.10696483333508973No Hit
GTCCACGAGGTCTCTTACTACTCACGATGACTCGGCGTACGCTGCA1990.10485715189006332No Hit
GTCCACGAGGTCTCTGAGTATATCATCTCACACCGCGTACGCTGCA1990.10485715189006332No Hit
GTCCACGAGGTCTCTGCGCACTACAAGGCTGACGTACGCTGCAGGT1980.10433023152880674No Hit
GTCCACGAGGTCTCTAGGGACCAGTCACAGTCATCCGTACGCTGCA1980.10433023152880674No Hit
GTCCACGAGGTCTCTCCTGATACGCATTGACGATACGTACGCTGCA1980.10433023152880674No Hit
GTCCACGAGGTCTCTATAGGCCGGTAATCCTGACGGTACGCTGCAG1970.10380331116755014No Hit
GTCCACGAGGTCTCTCATGAGTCGTGATACGCTTCGTACGCTGCAG1970.10380331116755014No Hit
GTCCACGAGGTCTCTGGGCACACTAGGAAACTCCACGTACGCTGCA1970.10380331116755014No Hit
GTCCACGAGGTCTCTGGTGCTCAGTCATCTTCACTCGTACGCTGCA1960.10327639080629353No Hit
GTCCACGAGGTCTCTCGATCCACAGTAGCTGAATCCGTACGCTGCA1960.10327639080629353No Hit
GTCCACGAGGTCTCTGATGCGGCACTATCGCTCATCGTACGCTGCA1940.10222255008378034No Hit
GTCCACGAGGTCTCTAGCGAGCACACATTCATTACCGTACGCTGCA1940.10222255008378034No Hit
GTCCACGAGGTCTCTATCTATATCTCAGGGAGGGCGTACGCTGCAG1940.10222255008378034No Hit
GTCCACGAGGTCTCTCCGATTTGCACAATGTCTCACGTACGCTGCA1940.10222255008378034No Hit
GTCCACGAGGTCTCTGCGACTTACAGCACTATCAGTGTACGCTGCA1920.10116870936126715No Hit
GTCCACGAGGTCTCTATAGCGAGCTTGAACACCGCCGTACGCTGCA1910.10064178900001054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA304.8460297E-640.00000422
CTTGTGT304.8460297E-640.00000432
TCTGCCT1100.040.00000413
TATTTCT304.8460297E-640.00000416
TTAGGTT304.8460297E-640.00000428
GAGGATA304.8460297E-640.00000423
AAACTCA304.8460297E-640.00000423
AAACTAG304.8460297E-640.00000425
CGGGTAA304.8460297E-640.00000421
TGATAGT304.8460297E-640.00000415
CGGCGGC600.040.0000042
GTACCGT600.040.00000432
GCCTTAC304.8460297E-640.00000422
GCAAGTT600.040.0000047
TACCCGA304.8460297E-640.00000426
AAATCGG304.8460297E-640.00000425
ACAAAAC600.040.00000419
TGCCCAT304.8460297E-640.00000415
TTGGATA304.8460297E-640.00000421
ATGATGG304.8460297E-640.00000417
Produced by FastQC (version 0.11.2)