FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_3.3510000004f380.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_3.3510000004f380.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135361
Sequences flagged as poor quality0
Sequence length46
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA2227916.45895051011739Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC17321.279541374546583No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA5920.43734901485656874RNA PCR Primer, Index 41 (96% over 28bp)
GTCGGCAGATTGCGATAAACGGTCACATTAAATTTAACCTGACTAT5410.3996719882388576No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA4530.3346606481926109No Hit
GCATTAACACCATCCTTCATGAACTTAATCCACTGTTCACCATAAA2500.1846913069495645No Hit
TTCAAGATTGCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTT2290.16917723716580108No Hit
ATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCG1940.14332045419286205No Hit
CTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTTCAAC1910.14110415850946728No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA1670.1233737930423091Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTCCGAGTTATTGACATGAGTGCGTACGCTGCA1640.1211574973589143No Hit
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA1620.1196799669033178RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA1550.11450861030872998No Hit
GTCCACGAGGTCTCTCGAGTAAGTAGAGCTGATTCCGTACGCTGCA1480.10933725371414219No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTCA257.758218E-540.00000414
CTTTTAG257.758218E-540.00000414
ATCCTCC257.758218E-540.00000430
AACCGTG257.758218E-540.00000427
AAACCAA257.758218E-540.00000412
TAAATTG257.758218E-540.00000424
TTGCAAA257.758218E-540.00000437
GTGTTCC257.758218E-540.00000430
TCTAGTT257.758218E-540.00000413
CGTTATG257.758218E-540.00000425
CCCAGGG257.758218E-540.00000429
TAAACCA257.758218E-540.00000411
TCTAGAC507.6397555E-1140.00000413
GATATTC257.758218E-540.00000430
ACATGCA257.758218E-540.00000419
AATTCGA257.758218E-540.00000427
CCAGCCG257.758218E-540.00000431
GATACAT257.758218E-540.00000416
CTCCCAG257.758218E-540.00000414
CTCCTAT257.758218E-540.00000414
Produced by FastQC (version 0.11.2)