FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_29.3510000004f521.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_29.3510000004f521.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178793
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA75414.217726644779158Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC32701.8289306628335562No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA6860.3836839249858775No Hit
CCGCATAAAGTGCACCGCATGGAAATGAAGACGGCCATTAGCTGTA3450.19296057451913665No Hit
TATTAATATCAAGTTGGGGGAGCACATTGTAGCATTGTGCCAATTC3430.19184196249293875No Hit
GTCCACGAGGTCTCTCATGAGTCGTGATACGCTTCGTACGCTGCAG3110.17394417007377247No Hit
TAACAGTCGGGAGAGGAGTGGCATTAACACCATCCTTCATGAACTT3110.17394417007377247No Hit
GTCCACGAGGTCTCTCGATCCACAGTAGCTGAATCCGTACGCTGCA2780.1554870716415072No Hit
TTCCTGCGCGTACACGCAAGGTAAACGCGAACAATTCAGCGGCTTT2610.14597886941882512No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA2610.14597886941882512No Hit
GTCCACGAGGTCTCTGGCCCGCACACAATTAGGAACGTACGCTGCA2600.14541956340572618No Hit
GTCCACGAGGTCTCTAGATGGCTGCTCATGCTCCTCGTACGCTGCA2540.14206372732713252No Hit
GTCCACGAGGTCTCTTTCTAACCCGCGAGTGCTTTCGTACGCTGCA2380.13311483111754935No Hit
GTCCACGAGGTCTCTGCAGTAAGCTAAGTCACCTCCGTACGCTGCA2270.12696246497346092No Hit
GTCCACGAGGTCTCTTAAGCTAGTATAACCGCTGCCGTACGCTGCA2270.12696246497346092No Hit
GTCCACGAGGTCTCTTAACGGAGGCGAACTTAGCCGTACGCTGCAG2250.12584385294726305No Hit
GTCCACGAGGTCTCTGAGATGTATCCCACCATATCCGTACGCTGCA2220.12416593490796618No Hit
GTCCACGAGGTCTCTGGATTATACCGACCACATTCCGTACGCTGCA2220.12416593490796618No Hit
GTCCACGAGGTCTCTGATGCGGCACTATCGCTCATCGTACGCTGCA2190.12248801686866935No Hit
GTCCACGAGGTCTCTCATCAGAGTCCATGTCAGGGCGTACGCTGCA2150.12025079281627357No Hit
GTCCACGAGGTCTCTACAGAGCTACAAGGTTTGGACGTACGCTGCA2090.11689495673767988No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA2050.11465773268528409RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTACAGCACTGCAATGACGGTACGTACGCTGCA2040.11409842667218514No Hit
GTCCACGAGGTCTCTAGTCGGATCACCCGCTGAATCGTACGCTGCA1980.11074259059359148No Hit
GTCCACGAGGTCTCTATCGTGACATGGCACATTCCGTACGCTGCAG1960.10962397856739357No Hit
GTCCACGAGGTCTCTTGGCATATCACAACAGTCCACGTACGCTGCA1930.10794606052809674No Hit
GTCCACGAGGTCTCTCCAGGACTTACATTCAGGAGTGTACGCTGCA1920.1073867545149978No Hit
GTCCACGAGGTCTCTAGATATACGACAGACCTCGCCGTACGCTGCA1860.10403091843640411No Hit
GTCCACGAGGTCTCTTTGTCATCCACCACGGTAGGTGTACGCTGCA1860.10403091843640411No Hit
GTCCACGAGGTCTCTCATCTGATAGCATTGCGGTGCGTACGCTGCA1850.10347161242330516No Hit
GTCCACGAGGTCTCTCTGAAGGAGCAATTTCGAGACGTACGCTGCA1840.10291230641020623No Hit
GTCCACGAGGTCTCTAATCGACTGTAAGTATCGCGCGTACGCTGCA1840.10291230641020623No Hit
GTCCACGAGGTCTCTGAGTCATGCACATTTCGGTCTGTACGCTGCA1830.10235300039710728No Hit
GTCCACGAGGTCTCTGCGGAGACATCAGACTAATCCGTACGCTGCA1830.10235300039710728No Hit
TCAAGATTGCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTG1800.10067508235781042No Hit
GTCCACGAGGTCTCTGGTGCTCAGTCATCTTCACTCGTACGCTGCA1800.10067508235781042No Hit
GTCCACGAGGTCTCTATAGGCGGACCACCTCTAAGCGTACGCTGCA1800.10067508235781042No Hit
GTCCACGAGGTCTCTGCTCATAACCAAGTGCCAGACGTACGCTGCA1800.10067508235781042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAA304.844451E-640.03
ACGCGAA353.0370938E-740.025
TCCGGCC257.768236E-540.018
AGCACCG200.001252926440.027
TCCGGAA304.844451E-640.015
TCTGCGA1800.040.013
TCTGCCT1000.040.013
GTCATTT200.001252926440.025
ATCCTTC257.768236E-540.032
GTTTGTT257.768236E-540.024
GTGTTGC304.844451E-640.030
CTTAAGA401.9126674E-840.014
TTCTATG257.768236E-540.015
TCGCCAA257.768236E-540.022
ACAACGC507.6397555E-1140.025
GTTGAAC257.768236E-540.024
CTAGATA507.6397555E-1140.014
ACAACAG304.844451E-640.025
AGCGTTT200.001252926440.016
TGTTTAC200.001252926440.030
Produced by FastQC (version 0.11.2)