FastQC Report![[OK]](data:image/png;base64,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)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | 000000000-AKRU9_l01n01_exp163gap1bff_28.3510000004f514.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 216188 |
| Sequences flagged as poor quality | 0 |
| Sequence length | 46 |
| %GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
![[FAIL]](data:image/png;base64,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)
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 87886 | 40.65258016171111 | Illumina PCR Primer Index 11 (96% over 28bp) |
| GCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA | 3271 | 1.5130349510611134 | Illumina PCR Primer Index 11 (96% over 28bp) |
| GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 2481 | 1.1476122634003736 | RNA PCR Primer, Index 41 (96% over 28bp) |
| GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC | 1264 | 0.5846763002571835 | No Hit |
| GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA | 1102 | 0.5097415212685256 | RNA PCR Primer, Index 41 (96% over 30bp) |
| GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA | 718 | 0.33211834144355834 | RNA PCR Primer, Index 41 (96% over 28bp) |
| GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA | 650 | 0.3006642366828871 | Illumina PCR Primer Index 11 (96% over 28bp) |
| GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA | 513 | 0.23729346679741706 | No Hit |
| GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGCAAAAAA | 457 | 0.21139008640627602 | Illumina PCR Primer Index 11 (96% over 28bp) |
| AGTATTGTTATCGGTAGCAAGCACATCACCTTGAATGCCACCGGAG | 353 | 0.16328380853701407 | No Hit |
| GTCCACGAGGTCGACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 310 | 0.14339371287953076 | RNA PCR Primer, Index 41 (96% over 28bp) |
| GTCCACAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA | 298 | 0.13784298851000054 | RNA PCR Primer, Index 41 (96% over 30bp) |
| GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA | 255 | 0.11795289285251725 | No Hit |
| GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCGTGAAAAAAA | 245 | 0.11332728921124206 | RNA PCR Primer, Index 41 (96% over 28bp) |
| AGGTAGCGTTGACCCTAATTTTGGTCGTCGGGTACGCAATCGCCGC | 244 | 0.11286472884711454 | No Hit |
| AAAGTCCAGCGTACCATAAACGCAAGCCTCAACGCAGCGACGAGCA | 219 | 0.10130071974392658 | No Hit |
Adapter Content
Kmer Content
| Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| CTCTGAT | 245 | 0.0 | 40.000004 | 12 |
| ACACGGT | 30 | 4.8491693E-6 | 40.000004 | 22 |
| CTGAGTT | 30 | 4.8491693E-6 | 40.000004 | 14 |
| AACAAGC | 30 | 4.8491693E-6 | 40.000004 | 24 |
| GCCCAGA | 30 | 4.8491693E-6 | 40.000004 | 16 |
| CTTTAAC | 30 | 4.8491693E-6 | 40.000004 | 19 |
| CTCCCTG | 30 | 4.8491693E-6 | 40.000004 | 14 |
| AACGATT | 30 | 4.8491693E-6 | 40.000004 | 23 |
| GAAACAT | 30 | 4.8491693E-6 | 40.000004 | 24 |
| TCTGGTA | 60 | 0.0 | 40.000004 | 13 |
| CCTGTCC | 30 | 4.8491693E-6 | 40.000004 | 30 |
| CTCTTTA | 60 | 0.0 | 40.000004 | 12 |
| TAACGAT | 30 | 4.8491693E-6 | 40.000004 | 22 |
| GCGAATC | 30 | 4.8491693E-6 | 40.000004 | 30 |
| ACCCGGT | 30 | 4.8491693E-6 | 40.000004 | 26 |
| ACTCCAC | 30 | 4.8491693E-6 | 40.000004 | 30 |
| CGGGCGT | 30 | 4.8491693E-6 | 40.000004 | 32 |
| CAGGGTT | 30 | 4.8491693E-6 | 40.000004 | 26 |
| ATAGTGA | 30 | 4.8491693E-6 | 40.000004 | 19 |
| TCTCGCC | 30 | 4.8491693E-6 | 40.000004 | 13 |