FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_26.3510000004f4f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_26.3510000004f4f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324061
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA87262.692702917043396Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC42051.2975952058408755No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA18160.5603883219517313No Hit
CTAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCAC4670.14410867089838025No Hit
ACCTTGCTGCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCACT4540.14009708048793282No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG4420.13639407395521214No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA4040.12466788660159661No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA3950.1218906317020561No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA3940.12158204782432938No Hit
CGAGAAATAAAAGTCTGAAACATGATTAAACTCCTAAGCAGAAAAC3720.11479320251434144No Hit
GTCCACGAGGTCTCTGGCCCGCACACAATTAGGAACGTACGCTGCA3620.1117073637370742No Hit
GTCCACGAGGTCTCTGTAGAACACACTGCCCCGTACGCTGCAGGTC3530.10893010883753368No Hit
TTACAGTATGCCCATCGCAGTTCGCTACACGCAGGACGCTTTTTCA3530.10893010883753368No Hit
GTCCACGAGGTCTCTCACGGTTATCAGATGTGGATCGTACGCTGCA3430.10584427006026643No Hit
GTCCACGAGGTCTCTCAGACGAGCTAATTCTATCCCGTACGCTGCA3410.10522710230481298No Hit
CTGTTGGTTTCTATGTGGCTAAATACGTTAACAAAAAGTCAGATAT3350.10337559903845264No Hit
GTCCACGAGGTCTCTTGTGTGTCAACAACCACCAACGTACGCTGCA3340.10306701516072592No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA3260.10059834413891212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAT600.040.00000423
TGATCGC304.8566853E-640.00000415
TCCGGCT304.8566853E-640.00000415
CTTAATT304.8566853E-640.00000429
CTTGTCC304.8566853E-640.00000414
ACAACGC600.040.00000425
GAGGATG304.8566853E-640.00000423
CAGGTGA304.8566853E-640.00000417
GAAATAG304.8566853E-640.00000424
AACAAGC600.040.00000424
GGAATAT304.8566853E-640.00000424
CAGAAAA600.040.00000439
CCTTAAT304.8566853E-640.00000428
GCCTTAA304.8566853E-640.00000427
AGATCTC304.8566853E-640.0000048
CAAACCG304.8566853E-640.00000431
GCAAGAA304.8566853E-640.00000424
TTAATTC304.8566853E-640.00000430
GTCGAGT304.8566853E-640.00000417
CAAGTGG304.8566853E-640.00000425