FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_25.3510000004f4ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_25.3510000004f4ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327208
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA80252.4525683968607126Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC51931.5870638859685582No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA14690.44894990342534413No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG4290.13110926383218013No Hit
ATTAATAATGTTTTCCGTAAATTCAGCGCCTTCCATGATGAGACAG4170.12744187183687442No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG4050.12377447984156867No Hit
GTCCACGAGGTCTCTAGATGTACCCAATTCTTCGACGTACGCTGCA4030.12316324784235105No Hit
GTCCACGAGGTCTCTACTAGCGCAGTGAGGTATCGTACGCTGCAGG4000.12224639984352462No Hit
GTCCACGAGGTCTCTGAAGAGTTCAAGCCATCCCCGTACGCTGCAG3610.11032737585878097No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA3550.1084936798611281No Hit
GTCCACGAGGTCTCTAGCTGGCATCGACTCTATCTCGTACGCTGCA3400.10390943986699593No Hit
GTCCACGAGGTCTCTTAGGTCATCACACGCAGTCCGTACGCTGCAG3390.1036038238673871No Hit
GTCCACGAGGTCTCTGATACATGCACAATGCCGCACGTACGCTGCA3360.10268697586856067No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA3330.10177012786973423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAG257.7824225E-540.028
TGATCGC200.001254452940.015
CGGACAA257.7824225E-540.022
TCCGGAA200.001254452940.015
TGATCAG257.7824225E-540.015
TCGCCTA257.7824225E-540.015
TATTTTT200.001254452940.013
ACAACTC200.001254452940.014
GTTTGTT451.2150849E-940.024
TGTTTTC451.2150849E-940.09
CTTAAGT401.9210347E-840.014
CTTAAGA200.001254452940.014
TCGCCCG507.6397555E-1140.031
ATTTACT257.7824225E-540.025
ACAACGC257.7824225E-540.025
GTGTTCC850.040.030
TTGATCA200.001254452940.015
TATTTAT200.001254452940.016
ATTTAAG200.001254452940.021
TATTTAA200.001254452940.019
Produced by FastQC (version 0.11.2)