000000000-AKRU9_l01n01_exp163gap1bff_24.3510000004f4df.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_24.3510000004f4df.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37133
Sequences flagged as poor quality	0
Sequence length	46
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	5224	14.068348907979425	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	364	0.980260145961813	No Hit
ACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAATGATTG	242	0.6517114157218646	No Hit
TGACTATTGACGTCCTTCCCCGTACGCCGGGCAATAATGTTTATGT	177	0.4766649610858266	No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA	136	0.3662510435461719	RNA PCR Primer, Index 41 (96% over 28bp)
CCGCGTTTCTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGCGC	119	0.32046966310290037	No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	86	0.2315999245953734	No Hit
GTCCACGAGGTCTCTCTACGTCGGCTCATAGTCGTCGTACGCTGCA	71	0.19120458891013384	No Hit
GTCCACGAGGTCTCTCGATCCACAGTAGCTGAATCCGTACGCTGCA	70	0.18851156653111786	No Hit
GTCCACGAGGTCTCTGCCAAAGACAGATGCCATTCCGTACGCTGCA	61	0.16427436511997415	No Hit
GTCCACGAGGTCTCTTCATCGGGAACATTTGCGCCCGTACGCTGCA	53	0.1427301860878464	No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG	50	0.13465111895079848	No Hit
GTCCACGAGGTCTCTGGGATCACAACCACGTTACACGTACGCTGCA	50	0.13465111895079848	No Hit
GTCCACGAGGTCTCTAAGGGCATCCAAGTTGTTGACGTACGCTGCA	49	0.1319580965717825	No Hit
GTCCACGAGGTCTCTAGACGGACTCGCATTCACTGCGTACGCTGCA	49	0.1319580965717825	No Hit
GTCCACGAGGTCTCTAGAGTCGCCCAACCGTTATACGTACGCTGCA	48	0.12926507419276653	No Hit
GTCCACGAGGTCTCTTCGCGGACCAGATTACTTAGCGTACGCTGCA	47	0.12657205181375056	No Hit
GTCCACGAGGTCTCTTCGTAGTACAGATCAGGTAGCGTACGCTGCA	46	0.1238790294347346	No Hit
GTCCACGAGGTCTCTAGATGGCTGCTCATGCTCCTCGTACGCTGCA	46	0.1238790294347346	No Hit
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA	46	0.1238790294347346	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTCACCAGAGCCGAGGATTCATCGTACGCTGCA	45	0.12118600705571864	No Hit
GTCCACGAGGTCTCTGAGTCGGCTACACTCTATGCGTACGCTGCAG	43	0.1157999622976867	No Hit
GTCCACGAGGTCTCTAGACACCTATTTGTAACGGGCGTACGCTGCA	43	0.1157999622976867	No Hit
GTCCACGAGGTCTCTCATGAGTCGTGATACGCTTCGTACGCTGCAG	43	0.1157999622976867	No Hit
GTCCACGAGGTCTCTCACATGGTCAAGGATGCCCCGTACGCTGCAG	42	0.11310693991867073	No Hit
GTCCACGAGGTCTCTTGGCATATCACAACAGTCCACGTACGCTGCA	42	0.11310693991867073	No Hit
GTCCACGAGGTCTCTCCGGTTGCCAAAGCGAGAAACGTACGCTGCA	41	0.11041391753965477	No Hit
GTCCACGAGGTCTCTGATAGATCCATCACGCTCCGTACGCTGCAGG	40	0.10772089516063879	No Hit
GTCCACGAGGTCTCTGCGCAGAAGGCAATGCTATACGTACGCTGCA	40	0.10772089516063879	No Hit
GTCCACGAGGTCTCTCCTAATGACCAAGGAGGCGACGTACGCTGCA	40	0.10772089516063879	No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA	40	0.10772089516063879	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTACTGACTGTGCAGGTTTAGTCGGACGCTGCA	40	0.10772089516063879	No Hit
GTCCACGAGGTCTCAACACAGGCAGGAATGTATCCGTACGCTGCAG	40	0.10772089516063879	No Hit
GTCCACGAGGTCTCTGAGTATATCATCTCACACCGCGTACGCTGCA	39	0.1050278727816228	No Hit
GTCCACGAGGTCTCTGAAGTGGTCGTCATACTCTCGTACGCTGCAG	39	0.1050278727816228	No Hit
GTCCACGAGGTCTCTCTCTTGGGAGAAGGAATACCGTACGCTGCAG	39	0.1050278727816228	No Hit
GTCCACGAGGTCTCTGGACTATACTCAATTCGACCCGTACGCTGCA	39	0.1050278727816228	No Hit
GTCCACGAGGTCTCTATTGTGCTCCCGCGCTTGTCGTACGCTGCAG	38	0.10233485040260684	No Hit
GTCCACGAGGTCTCTAGGCTTCACGCACCTTGGATCGTACGCTGCA	38	0.10233485040260684	No Hit
GTCCACGAGGTCTCTATAGAGTTGCCACCCTGTCGCGTACGCTGCA	38	0.10233485040260684	No Hit
GTCCACGAGGTCTCTGGGTACTAGACACTATCCTCCGTACGCTGCA	38	0.10233485040260684	No Hit
GTCCACGAGGTCTCTATAACTGGGTCGAACGGATCGTACGCTGCAG	38	0.10233485040260684	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTGGC	75	0.0	40.000004	12
CTCTTAC	75	0.0	40.000004	12
CTCTGGG	55	5.456968E-12	40.0	12
CTCTGGA	30	4.7412323E-6	40.0	12
CGTTTCT	20	0.0012401298	40.0	4
CTCTGCC	50	7.2759576E-11	40.0	12
CTCTGCA	40	1.8433639E-8	40.0	12
CTCTGAA	20	0.0012401298	40.0	12
GAGGTCG	650	0.0	40.0	7
GAGCGTA	55	5.456968E-12	40.0	33
TTGTTCC	20	0.0012401298	40.0	11
GGCACTA	20	0.0012401298	40.0	25
TGGCACT	20	0.0012401298	40.0	24
ACGCCGG	35	2.9511284E-7	40.0	24
GGCATGT	20	0.0012401298	40.0	24
CTATAGA	20	0.0012401298	40.0	14
GGGCAAT	35	2.9511284E-7	40.0	29
TCTAGCT	20	0.0012401298	40.0	13
TCTAGCC	25	7.6496035E-5	40.0	13
TCTAGAT	35	2.9511284E-7	40.0	13

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content