FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_23.3510000004f4c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_23.3510000004f4c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2021
Sequences flagged as poor quality0
Sequence length46
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGTGTTATTAATATCAAGTTGGGGGAGCACATTGTAGCATTGTG36317.96140524492825No Hit
GGAAACGTACGGATTGTTCAGTAACTTGACTCATGATTTCTTACCT35717.664522513607125No Hit
AGGAGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTA26913.310242454230577No Hit
GTTGAACACGACCAGAAAACTGGCCTAACGACGTTTGGTCAGTTCC25712.716476991588321No Hit
AACTTTACCGCTACTAAATGCCGCGGATTGGTTTCGCTGAATCAGG23211.479465611083622No Hit
GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGAACGTGATTAC1396.877783275606135No Hit
TTCGTATTCTGGCGTGAAGTCGCCGACTGAATGCCAGCAATCTCTT1336.580900544285008No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA190.9401286491835725Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC50.24740227610094012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTCT302.8582745E-640.0000047
CATGATT407.608833E-940.00000432
TTGTTCA407.608833E-940.00000414
CATTGTG302.8582745E-640.00000440
CATTGTA302.8582745E-640.00000432
AAACCAA302.8582745E-640.00000430
TCAAGTT302.8582745E-640.00000416
ACTAAAA302.8582745E-640.00000425
AACAACT302.8582745E-640.00000417
CACATTG302.8582745E-640.00000430
GTGTTAT302.8582745E-640.0000044
TAGTGTT302.8582745E-640.0000042
GAAACGT407.608833E-940.0000042
TTTCTTA407.608833E-940.00000437
ATGATTT407.608833E-940.00000433
TTGTAGC302.8582745E-640.00000434
GTCGCTA302.8582745E-640.00000440
TGACTCA407.608833E-940.00000427
ACTTGAC407.608833E-940.00000424
CAACTCA302.8582745E-640.00000419