Basic Statistics
Measure | Value |
---|---|
Filename | 000000000-AKRU9_l01n01_exp163gap1bff_23.3510000004f4c2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2021 |
Sequences flagged as poor quality | 0 |
Sequence length | 46 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
ATAGTGTTATTAATATCAAGTTGGGGGAGCACATTGTAGCATTGTG | 363 | 17.96140524492825 | No Hit |
GGAAACGTACGGATTGTTCAGTAACTTGACTCATGATTTCTTACCT | 357 | 17.664522513607125 | No Hit |
AGGAGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTA | 269 | 13.310242454230577 | No Hit |
GTTGAACACGACCAGAAAACTGGCCTAACGACGTTTGGTCAGTTCC | 257 | 12.716476991588321 | No Hit |
AACTTTACCGCTACTAAATGCCGCGGATTGGTTTCGCTGAATCAGG | 232 | 11.479465611083622 | No Hit |
GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGAACGTGATTAC | 139 | 6.877783275606135 | No Hit |
TTCGTATTCTGGCGTGAAGTCGCCGACTGAATGCCAGCAATCTCTT | 133 | 6.580900544285008 | No Hit |
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 19 | 0.9401286491835725 | Illumina PCR Primer Index 11 (96% over 28bp) |
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC | 5 | 0.24740227610094012 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GAGGTCT | 30 | 2.8582745E-6 | 40.000004 | 7 |
CATGATT | 40 | 7.608833E-9 | 40.000004 | 32 |
TTGTTCA | 40 | 7.608833E-9 | 40.000004 | 14 |
CATTGTG | 30 | 2.8582745E-6 | 40.000004 | 40 |
CATTGTA | 30 | 2.8582745E-6 | 40.000004 | 32 |
AAACCAA | 30 | 2.8582745E-6 | 40.000004 | 30 |
TCAAGTT | 30 | 2.8582745E-6 | 40.000004 | 16 |
ACTAAAA | 30 | 2.8582745E-6 | 40.000004 | 25 |
AACAACT | 30 | 2.8582745E-6 | 40.000004 | 17 |
CACATTG | 30 | 2.8582745E-6 | 40.000004 | 30 |
GTGTTAT | 30 | 2.8582745E-6 | 40.000004 | 4 |
TAGTGTT | 30 | 2.8582745E-6 | 40.000004 | 2 |
GAAACGT | 40 | 7.608833E-9 | 40.000004 | 2 |
TTTCTTA | 40 | 7.608833E-9 | 40.000004 | 37 |
ATGATTT | 40 | 7.608833E-9 | 40.000004 | 33 |
TTGTAGC | 30 | 2.8582745E-6 | 40.000004 | 34 |
GTCGCTA | 30 | 2.8582745E-6 | 40.000004 | 40 |
TGACTCA | 40 | 7.608833E-9 | 40.000004 | 27 |
ACTTGAC | 40 | 7.608833E-9 | 40.000004 | 24 |
CAACTCA | 30 | 2.8582745E-6 | 40.000004 | 19 |