000000000-AKRU9_l01n01_exp163gap1bff_22.3510000004f4b5.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_22.3510000004f4b5.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	444474
Sequences flagged as poor quality	0
Sequence length	46
%GC	52

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	198510	44.66177999163056	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA	4850	1.0911774367004594	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	2480	0.5579628954674515	No Hit
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA	1484	0.33387779712649107	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA	1381	0.3107043381615123	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA	1356	0.30507971219913876	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	1121	0.25220822815282784	No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGCAAAAAA	1029	0.23150960461129333	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG	680	0.15298982617655926	No Hit
GTCCACGAGGTCGACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	523	0.11766717513285367	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAC	473	0.10641792320810665	Illumina PCR Primer Index 11 (96% over 28bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGC	30	4.860767E-6	40.000004	28
TATTTCT	30	4.860767E-6	40.000004	16
ATCCTCC	30	4.860767E-6	40.000004	30
CAGAATA	30	4.860767E-6	40.000004	23
AAACTCT	30	4.860767E-6	40.000004	25
CGGAAAT	30	4.860767E-6	40.000004	27
TCGTTGG	30	4.860767E-6	40.000004	29
ATCTCCG	30	4.860767E-6	40.000004	31
GCAAGTC	30	4.860767E-6	40.000004	24
CAAACCG	60	0.0	40.000004	31
GGAGCCA	30	4.860767E-6	40.000004	21
TTGGATC	30	4.860767E-6	40.000004	30
GTATTCC	30	4.860767E-6	40.000004	21
CTGTAGT	30	4.860767E-6	40.000004	14
GTACAGG	30	4.860767E-6	40.000004	23
CTCAACC	30	4.860767E-6	40.000004	14
CAACGTG	30	4.860767E-6	40.000004	25
CAAGTAC	30	4.860767E-6	40.000004	25
GGTCGCG	30	4.860767E-6	40.000004	31
GGATGTT	30	4.860767E-6	40.000004	29