FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_21.3510000004f4a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_21.3510000004f4a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474
Sequences flagged as poor quality0
Sequence length46
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTTATAGGTCTGTTGAACACGACCAGAAAACTGGCCTAACGAC47119.037995149555375No Hit
TACAAACTCATCACGAACGTCAGAAGCAGCCTTATGGCCGTCAACA30212.206952303961197No Hit
TGTATCCATCTGAATGCAATGAAGAAAACCACCATTACCAGCATTA26110.549717057396927No Hit
ATCTCTTCCAAGAGCTTGATGCGGTTATCCATCTGCTTATGGAAGC25510.3071948261924No Hit
AACAACCTGATTAGCGGCGTTGACAGATGTATCCATCTGAATGCAA2439.822150363783347No Hit
AGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACACCAATCT1988.003233629749394No Hit
AGTGAGAACCAGCTTATCAGAAAAAAAGTTTGAATTATGGCGAGAA1465.901374292643492No Hit
CACCTAAAGCTACATCGTCAACGTTATATTTTGATAGTTTGACGGT1365.497170573969281No Hit
TTTGTCATTGTGAGCATTTTCATCCCGAAGTTGCGGCTCATTCTGA1335.375909458367016No Hit
ATCGTTAGTTGATGGCGAAAGGTCGCAAAGTAAGAGCTTCTCGAGC1275.13338722716249No Hit
CCTATAAACATTCTGTGCCGCGTTTCTTTGTTCCTGAGCATGGCAC1214.890864995957963No Hit
TACAACCTCATCACGAACGTCAGAAGCAGCCTTATGGCCGTCAACA30.12126111560226355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGCG200.001026294740.00000417
TAGCGGC200.001026294740.00000412
CAAGAGC200.001026294740.0000049
GTTGACA200.001026294740.00000419
ATCTCTT200.001026294740.0000041
AACAACC200.001026294740.0000041
AGCTTGA200.001026294740.00000413
ACAGATG200.001026294740.00000423
GGCGTTG200.001026294740.00000416
ATTAGCG200.001026294740.00000410
GACAGAT200.001026294740.00000422
CAACCTG200.001026294740.0000043
CATCTGC200.001026294740.00000430
GCGGTTA200.001026294740.00000421
GATGCGG200.001026294740.00000418
TTATCCA200.001026294740.00000425
GCGGCGT200.001026294740.00000414
TGATTAG200.001026294740.0000048
CTCTTCC200.001026294740.0000043
GATGTAT200.001026294740.00000426
Produced by FastQC (version 0.11.2)