FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_20.3510000004f49c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_20.3510000004f49c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152080
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA131518.647422409258285Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC20681.359810625986323No Hit
TCAGCGAAACCAATCCGCGGCATTTAGTAGCGGTAAAGTTAGACCA5770.3794055760126249No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA5020.3300894266175697No Hit
AAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATA3570.23474487112046294No Hit
AAAGGAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGC3260.21436086270384008No Hit
AAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTT2890.19003156233561283No Hit
TAGCGGCGTTGACAGATGTATCCATCTGAATGCAATGAAGAAAACC2790.18345607574960546No Hit
GAGTCGAAAAATTATCTTGATAAAGCAGGAATTACTACTGCTTGTT2740.18016833245660177No Hit
GAGGCCGTCCCTAGTTTTGTTTCTGGTGCTATGGCTAAAGCTGGTA2650.17425039452919516No Hit
TATTTTGACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATTGAGG2380.15649658074697528No Hit
AGCAGTCGGCGTGTGAATCATTAGCCTTGCGACCCTCGGCAGCAAG2260.14860599684376644No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA2210.14531825355076275RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTGTTGCACATAAACAGCTCCACGTACGCTGCA1960.12887953708574434No Hit
GTCCACGAGGTCTCTGATAGATCCATCACGCTCCGTACGCTGCAGG1950.1282219884271436No Hit
GCTTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAATCAAAAAG1950.1282219884271436No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA1900.12493424513413992No Hit
TGTCCAGTTGCATTTTAGTAAGCTCTTTTTGATTCTCAAATCCGGC1880.12361914781693845No Hit
GGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGAT1820.11967385586533404No Hit
GTCCACGAGGTCTCTAGATATTGCCAACCGGCCTACGTACGCTGCA1790.11770120988953182No Hit
GTCCACGAGGTCTCTAGATGGCTGCTCATGCTCCTCGTACGCTGCA1780.11704366123093109No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA1770.11638611257233035No Hit
ACCAATAAAATCCCTAAGCATTTGTTTCAGGGTTATTTGAATATCT1700.11178327196212519No Hit
GGTATTGGCTCTAATTTGTCTAGGAAATAACCGTCAGGATTGACAC1650.10849552866912152No Hit
GTCCACGAGGTCTCTTGTGTGTCAACAACCACCAACGTACGCTGCA1600.10520778537611783No Hit
TCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTAACCTTGCG1540.1012624934245134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCA200.001252335840.021
AGCACCG200.001252335840.031
CCCTGAT200.001252335840.017
TCACGCT257.76275E-540.026
TCTGCGG507.6397555E-1140.013
TTGATTT353.033092E-740.08
CTGCTTC257.76275E-540.031
TCTGCCT401.9093932E-840.013
CTTGTCC257.76275E-540.014
GTTTGTT257.76275E-540.024
CTTGTAT257.76275E-540.014
ATTTAGG304.839667E-640.018
GTTGAGA257.76275E-540.024
CTTAAGA200.001252335840.014
ACAACGG257.76275E-540.025
CTAGATA401.9093932E-840.014
TTTAGGG257.76275E-540.019
TTGATAA200.001252335840.017
GTTTGCT257.76275E-540.06
CTATGTT200.001252335840.014
Produced by FastQC (version 0.11.2)