FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_2.3510000004f373.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_2.3510000004f373.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15197
Sequences flagged as poor quality0
Sequence length46
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTGC3672.4149503191419357No Hit
AGACGAATCACCAGAACGGAAAACATCCTTCATAGAAATTTCACGC3502.3030861354214647No Hit
GGTCAGTATGCAAATTAGCATAAGCAGCTTGCAGACCCATAATGTC3252.1385799828913603No Hit
GCAAATCACCAGAAGGCGGTTCCTGAATGAATGGGAAGCCTTCAAG2631.7306047246167005No Hit
CTGCGTCAGTAAGAACGTCAGTGTTTCCTGCGCGTACACGCAAGGT2451.612160294795025No Hit
ATTACCCTTCTGAATGTCACGCTGATTATTTTGACTTTGAGCGTAT2441.605580048693821No Hit
TAGCTCCTAGACCTTTAGCAGCAAGGTCCATATCTGACTTTTTGTT2331.533197341580575No Hit
GGGAAGTAGCGACAGCTTGGTTTTTAGTGAGTTGTTCCATTCTTTA2301.5134566032769625No Hit
CGGTTCCGACTACCCTCCCGACTGCCTATGATGTTTATCCTTTGGA2211.4542343883661248No Hit
GCTGCCGAGGGTCGCAAGGCTAATGATTCACACGCCGACTGCTATC2141.4081726656576956No Hit
CGGCACCTGTTTTACAGACACCTAAAGCTACATCGTCAACGTTATA1991.3094689741396328No Hit
TTTGGGGTAATTATACTCATCGCGAATATCCTTAAGAGGGCGTTCA1911.2568270053299995No Hit
ACTGACGCAGAAGAAAACGTGCGTCAAAAATTACGTGCAGAAGGAG1831.204185036520366No Hit
GGCACGATTAACCCTGATACCAATAAAATCCCTAAGCATTTGTTTC1821.1976047904191618No Hit
CATTTGGAGGTAAAACCTCTTATGACGCTGACAACCGTCCTTTACT1721.1318023294071198No Hit
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC1661.0923208527998947No Hit
GAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTGATTTGCAAG1641.0791603605974862No Hit
ACCTATTAGTGGTTGAACAGCATCGGACTCAGATAGTAATCCACGC1551.0199381456866485No Hit
TGTTCCTGAGCATGGCACTATGTTTACTCTTGCGCTTGTTCGTTTT1380.9080739619661776No Hit
CATTGCATTCAGATGGATACATCTGTCAACGCCGCTAATCAGGTTG1230.8093702704481148No Hit
TGATTTGGTCATTGGTAAAATACTGACCAGCCGTTTGAGCTTGAGT1140.750148055537277No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA990.6514443640192144Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA570.3750740277686385No Hit
GTCCACGAGGTCTCTGGCTAGAACCAATTCCCAGACGTACGCTGCA220.14476541422649208No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA220.14476541422649208No Hit
GTCCACGAGGTCTCTGAATACTGCACAACGGCGCACGTACGCTGCA210.1381851681252879No Hit
GTCCACGAGGTCTCTGGGCGTCCAACAATGCACAACGTACGCTGCA200.1316049220240837No Hit
GTCCACGAGGTCTCTTCATGGCGTTAATGGCTATGCGTACGCTGCA200.1316049220240837No Hit
GTCCACGAGGTCTCTAACTCATGTGAATGCAGGGCCGTACGCTGCA200.1316049220240837No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG190.1250246759228795No Hit
GTCCACGAGGTCTCTACACGATGGTAAGATTTGCGCGTACGCTGCA190.1250246759228795No Hit
GTCCACGAGGTCTCTGCTAATGCACGTTATGGAAGCGTACGCTGCA190.1250246759228795No Hit
GTCCACGAGGTCTCTCTTGACAGGAGTGATAAACCGTACGCTGCAG190.1250246759228795No Hit
GTCCACGAGGTCTCTGAATATGCGCTCCCACTGTGCGTACGCTGCA190.1250246759228795No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA180.11844442982167534No Hit
GTCCACGAGGTCTCTGCGCGGTTTACATTATAGTGCGTACGCTGCA180.11844442982167534No Hit
GTCCACGAGGTCTCTGCCATATACTAAGACCTTGCCGTACGCTGCA180.11844442982167534No Hit
GTCCACGAGGTCTCTTTGTGTCATCGCGCCCAGTTCGTACGCTGCA180.11844442982167534No Hit
GTCCACGAGGTCTCTCTGAGAAGCGTGCATTAGATCGTACGCTGCA170.11186418372047115No Hit
GTCCACGAGGTCTCTACTAGCGCAGTGAGGTATCGTACGCTGCAGG170.11186418372047115No Hit
GTCCACGAGGTCTCTACTGATAACCCGTACTGGCCGTACGCTGCAG170.11186418372047115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCATA451.0604708E-940.028
CGCGAAT257.436156E-540.021
CTCTGCT257.436156E-540.012
CTCTGCG257.436156E-540.012
ATCCTCC200.00121696240.030
CTCTGAT304.556923E-640.012
TAGAAAT451.0604708E-940.033
CTCTGAG451.0604708E-940.012
CGCTGAT600.040.020
AACGTTA257.436156E-540.038
TTTGACT600.040.030
ACTGCCT200.00121696240.021
TTTTGAC600.040.029
ATGGGAA304.556923E-640.031
ACTCGTA200.00121696240.033
TTGTTCG257.436156E-540.036
GTGTTTC257.436156E-540.021
TTCACGC451.0604708E-940.040
AACGTCA257.436156E-540.014
TCCTTCA451.0604708E-940.026
Produced by FastQC (version 0.11.2)