000000000-AKRU9_l01n01_exp163gap1bff_19.3510000004f48f.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_19.3510000004f48f.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	172982
Sequences flagged as poor quality	0
Sequence length	46
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	18268	10.560636366789607	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	2264	1.3088066966505185	No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	767	0.44339873512851047	No Hit
ACCAGAACGGAAAACATCCTTCATAGAAATTTCACGCGGCGGCAAG	682	0.3942606745210484	No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA	497	0.287313130845984	RNA PCR Primer, Index 41 (96% over 28bp)
TCTTTAGTCGCAGTAGGCGGAAAACGAACAAGCGCAAGAGTAAACA	335	0.19366176827646808	No Hit
AGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGA	298	0.17227225954145517	No Hit
ATTAGCCTTGCGACCCTCGGCAGCAAGAACCATACGACCAATATCA	273	0.15781988877455458	No Hit
AAAAAGAGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGC	265	0.1531951301291464	No Hit
GCGAGTGGTCGGCAGATTGCGATAAACGGTCACATTAAATTTAACC	259	0.14972656114509025	No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA	258	0.14914846631441422	No Hit
AGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATC	225	0.13007133690210543	No Hit
GTCCACGAGGTCTCTGGGCGTCCAACAATGCACAACGTACGCTGCA	223	0.12891514724075337	No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA	221	0.1277589575794013	No Hit
TCTTCCAAGAGCTTGATGCGGTTATCCATCTGCTTATGGAAGCCAA	218	0.12602467308737325	No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA	204	0.11793134545790893	No Hit
GTCCACGAGGTCTCTCTTGACAGGAGTGATAAACCGTACGCTGCAG	199	0.11504087130452878	No Hit
GTCCACGAGGTCTCTCATTGGGTGAAAGTGTCTCACGTACGCTGCA	197	0.11388468164317675	No Hit
GTCCACGAGGTCTCTAGCTGGCATCGACTCTATCTCGTACGCTGCA	195	0.1127284919818247	No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG	193	0.11157230232047265	No Hit
GTCCACGAGGTCTCTGAATATGCGCTCCCACTGTGCGTACGCTGCA	193	0.11157230232047265	No Hit
GTCCACGAGGTCTCTTGCAGTAAGCTCAATCACACCGTACGCTGCA	192	0.11099420748979663	No Hit
GTCCACGAGGTCTCTGGTATGACTATCACCCATCGCGTACGCTGCA	182	0.1052132591830364	No Hit
GTCCACGAGGTCTCTCCCGCAGACAGAATCATTTACGTACGCTGCA	180	0.10405706952168432	No Hit
GTCCACGAGGTCTCTACTAGCGCAGTGAGGTATCGTACGCTGCAGG	175	0.10116659536830422	No Hit
GTCCACGAGGTCTCTATTATAGTGCCAGACGTACGCTGCAGGTCGA	174	0.10058850053762819	No Hit
GTCCACGAGGTCTCTGCGGCCAATAGTAAACTTCACGTACGCTGCA	174	0.10058850053762819	No Hit
GTCCACGAGGTCTCTAGATGTACCCAATTCTTCGACGTACGCTGCA	173	0.10001040570695217	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTTC	30	4.843536E-6	40.000004	22
CTTAAGT	30	4.843536E-6	40.000004	33
ACAACGT	30	4.843536E-6	40.000004	32
TGTTTCA	30	4.843536E-6	40.000004	23
AGCGTGG	30	4.843536E-6	40.000004	19
CGTGGAA	30	4.843536E-6	40.000004	21
GAGTGCG	30	4.843536E-6	40.000004	31
TTGGCGT	30	4.843536E-6	40.000004	32
TTGGCCG	30	4.843536E-6	40.000004	31
TCTTGGT	30	4.843536E-6	40.000004	13
AGTCCAT	30	4.843536E-6	40.000004	22
AAATCGG	30	4.843536E-6	40.000004	25
TACCCAA	30	4.843536E-6	40.000004	21
TGCCCAA	30	4.843536E-6	40.000004	15
GCAAGAT	30	4.843536E-6	40.000004	21
GAGCTTT	30	4.843536E-6	40.000004	26
TTGGATC	30	4.843536E-6	40.000004	30
TCCGCAT	30	4.843536E-6	40.000004	15
GTATTCG	30	4.843536E-6	40.000004	31
CAAGTGG	30	4.843536E-6	40.000004	25

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content