000000000-AKRU9_l01n01_exp163gap1bff_18.3510000004f472.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_18.3510000004f472.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	101219
Sequences flagged as poor quality	0
Sequence length	46
%GC	56

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	1549	1.5303450933125204	No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	1199	1.184560211027574	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	387	0.38233928412649804	No Hit
TGAACACGACCAGAAAACTGGCCTAACGACGTTTGGTCAGTTCCAT	340	0.3359053142196623	No Hit
CTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTATGT	228	0.22525415188847944	No Hit
CCACGGAGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGAC	216	0.2133986702101384	No Hit
GTCCACGAGGTCTCTTGTGTGTCAACAACCACCAACGTACGCTGCA	206	0.20351910214485422	No Hit
TACTGAGAAGTTAATGGATGAATTGGCACAATGCTACAATGTGCTC	202	0.19956727491874057	No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA	188	0.1857358796273427	No Hit
GTCCACGAGGTCTCTAGCTGGCATCGACTCTATCTCGTACGCTGCA	174	0.17190448433594482	No Hit
AAAACAGGTGCCGAAGAAGCTGGAGTAACAGAAGTGAGAACCAGCT	174	0.17190448433594482	No Hit
GTCCACGAGGTCTCTTACATACTAAGAGAGCCGCCCGTACGCTGCA	169	0.16696470030330274	No Hit
GTCCACGAGGTCTCTCGAGTAAGTAGAGCTGATTCCGTACGCTGCA	164	0.16202491627066065	No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG	154	0.15214534820537645	No Hit
GTCCACGAGGTCTCTGGATAGTCCCAACCCTAATACGTACGCTGCA	153	0.15115739139884804	No Hit
TACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCTAAAGCTACA	144	0.14226578014009228	No Hit
CAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTTGG	139	0.1373259961074502	No Hit
GTCCACGAGGTCTCTGAAGAGTTCAAGCCATCCCCGTACGCTGCAG	139	0.1373259961074502	No Hit
GTCCACGAGGTCTCTGGGCGTCCAACAATGCACAACGTACGCTGCA	135	0.13337416888133652	No Hit
GTCCACGAGGTCTCTGCCCGACTGAAATAGGTACACGTACGCTGCA	135	0.13337416888133652	No Hit
GTCCACGAGGTCTCTAAGGTACTGGAAGCTACTGCCGTACGCTGCA	134	0.13238621207480808	No Hit
GTCCACGAGGTCTCTTGTCCGGCCAAAGAGCCAAACGTACGCTGCA	130	0.1284343848486944	No Hit
GTCCACGAGGTCTCTAGATGTACCCAATTCTTCGACGTACGCTGCA	129	0.127446428042166	No Hit
GTCCACGAGGTCTCTACGAACGTGGCATGTTTGATCGTACGCTGCA	128	0.12645847123563758	No Hit
GTCCACGAGGTCTCTACTAGCGCAGTGAGGTATCGTACGCTGCAGG	126	0.12448255762258074	No Hit
GTCCACGAGGTCTCTGGCTAGAACCAATTCCCAGACGTACGCTGCA	125	0.12349460081605232	No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA	124	0.12250664400952391	No Hit
GCCGCGTTTCTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGCG	124	0.12250664400952391	No Hit
GTCCACGAGGTCTCTCCTGACTACCAATGCAAGGACGTACGCTGCA	122	0.12053073039646706	No Hit
GTCCACGAGGTCTCTGCGATCAGAACAGATGCCGACGTACGCTGCA	119	0.11756685997688181	No Hit
GTCCACGAGGTCTCTAGGGACCATAGACCATTATCCGTACGCTGCA	119	0.11756685997688181	No Hit
GTCCACGAGGTCTCTTAGGTCATCACACGCAGTCCGTACGCTGCAG	119	0.11756685997688181	No Hit
GTCCACGAGGTCTCTCAGACGAGCTAATTCTATCCCGTACGCTGCA	118	0.11657890317035338	No Hit
GTCCACGAGGTCTCTTATATCGCACCAGGGCTTCCGTACGCTGCAG	118	0.11657890317035338	No Hit
GTCCACGAGGTCTCTGGAGTACCAACATATCCCGACGTACGCTGCA	116	0.11460298955729656	No Hit
GTCCACGAGGTCTCTGGTACAAGCAACGATCTCCACGTACGCTGCA	116	0.11460298955729656	No Hit
TGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGCCTGT	116	0.11460298955729656	No Hit
GTCCACGAGGTCTCTGCGCCACAGCACTTAATATACGTACGCTGCA	115	0.11361503275076815	No Hit
GTCCACGAGGTCTCTAATTGACCGCAGGCATCGGCGTACGCTGCAG	115	0.11361503275076815	No Hit
GTCCACGAGGTCTCTGAATATGCGCTCCCACTGTGCGTACGCTGCA	114	0.11262707594423972	No Hit
GTCCACGAGGTCTCTACAGTATGGACAGTTCCGCGCGTACGCTGCA	113	0.1116391191377113	No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA	113	0.1116391191377113	No Hit
GTCCACGAGGTCTCTTATGTTACGCGCCAGCGCGTACGCTGCAGGT	112	0.11065116233118287	No Hit
GTCCACGAGGTCTCTGTTGCACATAAACAGCTCCACGTACGCTGCA	111	0.10966320552465447	No Hit
GTCCACGAGGTCTCTCACTATACGTTGAGACCATGCGTACGCTGCA	110	0.10867524871812605	No Hit
GTCCACGAGGTCTCTCAACTTGTAGGGACGATGATCGTACGCTGCA	109	0.10768729191159762	No Hit
GTCCACGAGGTCTCTAGATGGCTGCTCATGCTCCTCGTACGCTGCA	109	0.10768729191159762	No Hit
GTCCACGAGGTCTCTATGACAAGAGGGTCGAACTCCGTACGCTGCA	108	0.1066993351050692	No Hit
GTCCACGAGGTCTCTAGATATTGCCAACCGGCCTACGTACGCTGCA	108	0.1066993351050692	No Hit
GTCCACGAGGTCTCTTACCTAACGACATTTGTGGCCGTACGCTGCA	108	0.1066993351050692	No Hit
GTCCACGAGGTCTCTCCTGCTGCCAAAGGATCAAACGTACGCTGCA	108	0.1066993351050692	No Hit
GTCCACGAGGTCTCTCAACTTCCGGTAATATCCTGCGTACGCTGCA	108	0.1066993351050692	No Hit
TGATAAGCAAGCATCTCATTTTGTGCATATACCTGGTCTTTCGTAT	108	0.1066993351050692	No Hit
GTCCACGAGGTCTCTCTTGACAGGAGTGATAAACCGTACGCTGCAG	107	0.10571137829854078	No Hit
GTCCACGAGGTCTCTGGTATGACTATCACCCATCGCGTACGCTGCA	106	0.10472342149201237	No Hit
GTCCACGAGGTCTCTATCTGCGACGGAGTCACGATTGTACGCTGCA	106	0.10472342149201237	No Hit
GTCCACGAGGTCTCTCAGACACCTGAATTTGTAGCCGTACGCTGCA	106	0.10472342149201237	No Hit
GTCCACGAGGTCTCTGCGAGGTAGACATTAGATTCGGTACGCTGCA	105	0.10373546468548395	No Hit
GTCCACGAGGTCTCTAACTCATGTGAATGCAGGGCCGTACGCTGCA	105	0.10373546468548395	No Hit
GTCCACGAGGTCTCTATATCGAGTGCGACATCCCGCGTACGCTGCA	105	0.10373546468548395	No Hit
GTCCACGAGGTCTCTCATCTCGTAGGATCTGGCTCGTACGCTGCAG	104	0.10274750787895554	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGAAC	30	4.8235943E-6	40.000004	35
CTCTGGT	120	0.0	40.000004	12
CTTCACT	30	4.8235943E-6	40.000004	14
AAACTGG	30	4.8235943E-6	40.000004	15
GTTTGGT	30	4.8235943E-6	40.000004	31
CCAACGT	30	4.8235943E-6	40.000004	32
CTATAGC	55	5.456968E-12	40.000004	14
ACAGAAG	30	4.8235943E-6	40.000004	28
GAAATAG	30	4.8235943E-6	40.000004	24
ATAAGCC	30	4.8235943E-6	40.000004	30
AGAAGCT	30	4.8235943E-6	40.000004	15
TGCGCAT	30	4.8235943E-6	40.000004	15
ACTGAAA	30	4.8235943E-6	40.000004	21
AGTAGCG	30	4.8235943E-6	40.000004	31
TGCCGAA	30	4.8235943E-6	40.000004	9
TCTGGGT	30	4.8235943E-6	40.000004	13
GTTCCGC	30	4.8235943E-6	40.000004	28
CTCTCTC	60	0.0	40.000004	12
CCGCCGT	30	4.8235943E-6	40.000004	32
AGAGGGC	30	4.8235943E-6	40.000004	30

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content