FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_17.3510000004f465.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_17.3510000004f465.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145683
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC25891.7771462696402462No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA7870.540214026344872Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA7180.4928509160300103No Hit
TTTTGCAAGCTATTTAACTGGCGGCGATTGCGTACCCGACGACCAA4880.334973881647138No Hit
TTTAGCCACATAGAAACCAACAGCCATATAACTGGTAGCTTTAAGC4210.2889836151095186No Hit
ATTTTTGACGCACGTTTTCTTCTGCGTCAGTAAGAACGTCAGTGTT3140.2155364730270519No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA2780.19082528503668925No Hit
GTCCACGAGGTCTCTAGCTGGCATCGACTCTATCTCGTACGCTGCA2680.18396106615047741No Hit
GTCCACGAGGTCTCTACTAGCGCAGTGAGGTATCGTACGCTGCAGG2460.16885978460081133No Hit
CCTTCAAGAAGTCCTTTACCAGCTTTAGCCATAGCACCAGAAACAA2450.16817336271219016No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA2390.16405483138046306No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA2300.1578770343828724No Hit
GTCCACGAGGTCTCTGCCATATACTAAGACCTTGCCGTACGCTGCA2120.1455214403876911No Hit
GTCCACGAGGTCTCTCGAGTAAGTAGAGCTGATTCCGTACGCTGCA2110.14483501849906988No Hit
GTCCACGAGGTCTCTTACATACTAAGAGAGCCGCCCGTACGCTGCA2110.14483501849906988No Hit
GTCCACGAGGTCTCTACAGTTGCGAAAGCTCCTCACGTACGCTGCA2100.1441485966104487No Hit
GTCCACGAGGTCTCTTCATGGCGTTAATGGCTATGCGTACGCTGCA2060.14140290905596398No Hit
GTCCACGAGGTCTCTTGTGTGTCAACAACCACCAACGTACGCTGCA2000.13728437772423688No Hit
GTCCACGAGGTCTCTGGGCGTCCAACAATGCACAACGTACGCTGCA1960.13453869016975215No Hit
GTCCACGAGGTCTCTAATTTGTGCGAAGCCGCTCCCGTACGCTGCA1940.13316584639250978No Hit
GTCCACGAGGTCTCTCCGAGTTATTGACATGAGTGCGTACGCTGCA1930.13247942450388858No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG1930.13247942450388858No Hit
GTCCACGAGGTCTCTTATTTCCAGCCAGGGTAGCTTACGCTGCAGG1880.12904731506078265No Hit
GTCCACGAGGTCTCTCCTATGCACTGATTCCAGATCGTACGCTGCA1870.12836089317216146No Hit
GTCCACGAGGTCTCTGCCCATGCTATAGATGATCCGTACGCTGCAG1860.1276744712835403No Hit
GTCCACGAGGTCTCTGGTATGACTATCACCCATCGCGTACGCTGCA1820.12492878372905554No Hit
GTCCACGAGGTCTCTATGACGTGTCGATCACTCCTCGTACGCTGCA1820.12492878372905554No Hit
GTCCACGAGGTCTCTGCCCAGGTTTAATAATCCTCCGTACGCTGCA1820.12492878372905554No Hit
GTCCACGAGGTCTCTCTTGACAGGAGTGATAAACCGTACGCTGCAG1800.12355593995181319No Hit
GTCCACGAGGTCTCTCACTTGACGCAAGGTTTATACGTACGCTGCA1780.12218309617457082No Hit
GTCCACGAGGTCTCTTAGGTCATCACACGCAGTCCGTACGCTGCAG1770.12149667428594964No Hit
GTCCACGAGGTCTCTGAGATAAGGAATGCCACCCCGTACGCTGCAG1730.11875098673146489No Hit
GTCCACGAGGTCTCTACATGCGTTAAATCCTCGGACGTACGCTGCA1730.11875098673146489No Hit
GTCCACGAGGTCTCTTGTCCGGCCAAAGAGCCAAACGTACGCTGCA1720.11806456484284372No Hit
GTCCACGAGGTCTCTCCATGTAACTCAAGTCAGACCGTACGCTGCA1690.11600529917698016No Hit
GTCCACGAGGTCTCTCATCTCGTAGGATCTGGCTCGTACGCTGCAG1660.1139460335111166No Hit
GTCCACGAGGTCTCTACCCAGTAGTGAACAGGAGCCGTACGCTGCA1620.11120034595663186No Hit
GTCCACGAGGTCTCTCCTAGGTATCAACTCAGACGTACGCTGCAGG1610.1105139240680107No Hit
GTCCACGAGGTCTCTTCACGAGTACAGCTACGCGCGTACGCTGCAG1610.1105139240680107No Hit
GTCCACGAGGTCTCTCATCAGGAAGCAAGCCTTTGACGTACGCTGC1600.1098275021793895No Hit
GTCCACGAGGTCTCTACCTTACAGTTTACAGAGGGCGTACGCTGCA1590.10914108029076831No Hit
GTCCACGAGGTCTCTGATGACAGTACAGTTGACAGCGTACGCTGCA1590.10914108029076831No Hit
GTCCACGAGGTCTCTGTTGCACATAAACAGCTCCACGTACGCTGCA1580.10845465840214712No Hit
GTCCACGAGGTCTCTGCAGATAGCACAATCGGCCACGTACGCTGCA1580.10845465840214712No Hit
GTCCACGAGGTCTCTGCGGCTGCAATACAGTTCTACGTACGCTGCA1580.10845465840214712No Hit
GTCCACGAGGTCTCTGAAGAGTTCAAGCCATCCCCGTACGCTGCAG1560.10708181462490476No Hit
GTCCACGAGGTCTCTTCAGGTGAGTCATTGGCTCTCGTACGCTGCA1550.10639539273628357No Hit
GTCCACGAGGTCTCTAACTCATGTGAATGCAGGGCCGTACGCTGCA1550.10639539273628357No Hit
GTCCACGAGGTCTCTCATCGGCATGGAGCTTAGATCGTACGCTGCA1530.10502254895904121No Hit
GTCCACGAGGTCTCTGCCATTATAGCAATCTGCACCGTACGCTGCA1530.10502254895904121No Hit
GTCCACGAGGTCTCTGAGCTGAACTAATAGCGAGCCGTACGCTGCA1530.10502254895904121No Hit
GTCCACGAGGTCTCTACGAACGTGGCATGTTTGATCGTACGCTGCA1520.10433612707042002No Hit
GTCCACGAGGTCTCTACTTTACATTGGGCTAAGGGCGTACGCTGCA1500.10296328329317764No Hit
GTCCACGAGGTCTCTCCTGATGAGTAATGCGATAGCGTACGCTGCA1500.10296328329317764No Hit
GTCCACGAGGTCTCTAGATGTACCCAATTCTTCGACGTACGCTGCA1470.10090401762731409No Hit
GTCCACGAGGTCTCTTTCATCGCGCTCATGTGGTGCGTACGCTGCA1460.10021759573869292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCAT353.031928E-740.015
TGATCGT507.6397555E-1140.032
TCCGGCT304.838259E-640.015
CTTGTGT304.838259E-640.014
TCTGCCT700.040.013
CCTTCCG507.6397555E-1140.031
GTTTGTT200.001252162240.024
CTTGTAT200.001252162240.014
TATCATG353.031928E-740.015
CTTAAGC257.761135E-540.014
CTTAAGA257.761135E-540.014
TCGCCCG304.838259E-640.031
ACAACGT257.761135E-540.032
TATTTCC304.838259E-640.016
ACAACGC200.001252162240.025
ATCCTGT200.001252162240.028
TATTTAA304.838259E-640.011
ATTTAAC304.838259E-640.012
CTTAAAC257.761135E-540.022
TTTAGGA200.001252162240.020
Produced by FastQC (version 0.11.2)