000000000-AKRU9_l01n01_exp163gap1bff_16.3510000004f459.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_16.3510000004f459.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	80922
Sequences flagged as poor quality	0
Sequence length	46
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	19763	24.422283186278143	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	536	0.6623662292083734	No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA	480	0.5931637873507822	RNA PCR Primer, Index 41 (96% over 28bp)
TGCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCACTAAAAACC	433	0.5350831665060182	No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	389	0.4807098193321964	No Hit
AAAATACGTGGCCTTATGGTTACAGTATGCCCATCGCAGTTCGCTA	369	0.45599466152591384	No Hit
TGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGAT	368	0.45475890363559973	No Hit
AGACCCATAATGTCAATAGATGTGGTAGAAGTCGTCATTTGGCGAG	311	0.38432070388769435	No Hit
GTGTACGCGCAGGAAACACTGACGTTCTTACTGACGCAGAAGAAAA	300	0.3707273670942389	No Hit
AAATCACCAGAAGGCGGTTCCTGAATGAATGGGAAGCCTTCAAGAA	295	0.36454857764266824	No Hit
TACCGATAACAATACTGTAGGCATGGGTGATGCTGGTATTAAATCT	255	0.31511826203010307	No Hit
GACCCTCGGCAGCAAGAACCATACGACCAATATCACGAAAATAGTC	244	0.30152492523664765	No Hit
TGCCGAGGGTCGCAAGGCTAATGATTCACACGCCGACTGCTATCAG	229	0.2829885568819357	No Hit
TGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCC	226	0.2792812832109933	No Hit
CACCAAGTCCAACCAAATCAAGCAACTTATCAGAAACGGCAGAAGT	226	0.2792812832109933	No Hit
TTATCAACAGAAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAA	221	0.27310249375942264	No Hit
GCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAAC	215	0.26568794641753785	No Hit
GGCAAAACTGCGTAACCGTCTTCTCGTTCTCTAAAAACCATTTTTC	203	0.2508588517337683	No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA	180	0.22243642025654337	Illumina PCR Primer Index 11 (96% over 28bp)
TATTGACTCTACTGTAGACATTTTTACTTTTTATGTCCCTCATCGT	178	0.2199649044759151	No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG	170	0.21007884135340205	No Hit
GTTTATGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC	170	0.21007884135340205	No Hit
GCTTGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTCAGTACCTT	165	0.20390005190183141	No Hit
GAGTCATTTCTTTGATTTGGTCATTGGTAAAATACTGACCAGCCGT	148	0.1828921677664912	No Hit
CTTTAGTACCTCGCAACGGCTGCGGACGACCAGGGCGAGCGCCAGA	145	0.1791848940955488	No Hit
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA	145	0.1791848940955488	RNA PCR Primer, Index 41 (96% over 28bp)
TGGTCAGATTGGTCGTCTTATTACCATTTCAACTACTCCGGTTATC	131	0.16188428363115098	No Hit
GTCCACGAGGTCTCTCAGACGAGCTAATTCTATCCCGTACGCTGCA	126	0.15570549417958035	No Hit
GTCCACGAGGTCTCTATGACGTGTCGATCACTCCTCGTACGCTGCA	125	0.15446973628926622	No Hit
CGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTC	121	0.1495267047280097	No Hit
GTCCACGAGGTCTCTACATGCGTTAAATCCTCGGACGTACGCTGCA	108	0.133461852153926	No Hit
GTCCACGAGGTCTCTAGATGGCTGCTCATGCTCCTCGTACGCTGCA	103	0.12728306270235534	No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGCAAAAAA	101	0.12481154692172709	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGGCCAGAGACAACGACCCACGTACGCTGCAG	101	0.12481154692172709	No Hit
GTCCACGAGGTCTCTCCGGAAGGTGTTGTAACTATCGTACGCTGCA	87	0.10751093645732929	No Hit
GTCCACGAGGTCTCTGGGATATAACTAAGGCTTCCCGTACGCTGCA	86	0.10627517856701515	No Hit
GTCCACGAGGTCTCTAGTCGGATCACCCGCTGAATCGTACGCTGCA	86	0.10627517856701515	No Hit
GTCCACGAGGTCTCTCCCGTAGCCTAAGTTTTCCGTACGCTGCAGG	85	0.10503942067670102	No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA	85	0.10503942067670102	No Hit
GTCCACGAGGTCTCTGGTACAAGCAACGATCTCCACGTACGCTGCA	82	0.10133214700575864	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTCGC	30	4.8115635E-6	40.000004	7
TCTAGAC	30	4.8115635E-6	40.000004	13
GCAAGGC	30	4.8115635E-6	40.000004	12
AAATCAC	30	4.8115635E-6	40.000004	1
CTCCTCG	30	4.8115635E-6	40.000004	31
TTCAAGA	30	4.8115635E-6	40.000004	39
TTCAACT	30	4.8115635E-6	40.000004	28
GTCGCGT	30	4.8115635E-6	40.000004	32
GTTCCTG	30	4.8115635E-6	40.000004	17
GGACCGT	30	4.8115635E-6	40.000004	32
GTCGTCT	30	4.8115635E-6	40.000004	12
GTCGCAA	30	4.8115635E-6	40.000004	9
AATCACC	30	4.8115635E-6	40.000004	2
TCTGGGT	30	4.8115635E-6	40.000004	13
TCTGGGC	30	4.8115635E-6	40.000004	13
CTCTTTC	30	4.8115635E-6	40.000004	12
AGCTAAT	30	4.8115635E-6	40.000004	22
GCGGTTC	30	4.8115635E-6	40.000004	14
GAAGCCT	30	4.8115635E-6	40.000004	33
CTCTTCG	60	0.0	40.000004	12

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content