000000000-AKRU9_l01n01_exp163gap1bff_15.3510000004f44c.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_15.3510000004f44c.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	470228
Sequences flagged as poor quality	0
Sequence length	46
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	113414	24.118938047075037	Illumina PCR Primer Index 11 (96% over 28bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA	19928	4.23794414624395	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC	7217	1.5347873797391904	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA	4108	0.8736187551570728	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAT	3937	0.8372534174910894	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCTCACGCTCTTCCGATCTGCAGGTCGACATCTCGT	3711	0.7891916261898483	No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA	3216	0.6839235434725283	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA	3070	0.652874775640753	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCTCTCGCTCTTCCGATCTGCAGGTCGACATCTCGT	2952	0.6277805660232908	No Hit
GTCCACGACGCTCTTCCGATCTGCAGGTCGACATCTCGTATGCCGT	2722	0.5788681235485764	No Hit
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAG	2315	0.49231436664766876	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACC	2264	0.4814685641858843	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	1730	0.3679066325271996	No Hit
GTCCACGAGGTCTCTGCTCTTCCGATCTGCAGGTCGACATCTCGTA	1528	0.32494874826679826	No Hit
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAA	1526	0.32452342268006157	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGCTCTTCCGATCTGCAGGTCGACATCTCGTATGCCGT	1436	0.30538377127691246	No Hit
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTGCA	1433	0.30474578289680754	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCTCTTCCGATCTGCAGGTCGACATCTCGTATGCCG	1428	0.3036824689299659	No Hit
GTCCACGAGGTCTACGCTCTTCCGATCTGCAGGTCGACATCTCGTA	1381	0.2936873176416547	No Hit
GTCCACGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	1361	0.2894340617742882	RNA PCR Primer, Index 31 (96% over 31bp)
GTCCACGAGGTCTCTCTTCCGATCTGCAGGTCGACATCTCGTATGC	1352	0.2875200966339733	No Hit
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTACA	1337	0.28433015473344847	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAATA	1254	0.2666791428838776	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAACA	1237	0.2630638753966161	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATAA	1219	0.2592359451159863	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATACA	1213	0.25795996835577634	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTTCC	1176	0.2500914450011484	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTTCA	1146	0.24371156120009868	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCTCTTCTTCCGATCTGCAGGTCGACATCTCGTATG	1139	0.2422229216465204	No Hit
GTCCACGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	1112	0.23648102622557568	RNA PCR Primer, Index 31 (96% over 31bp)
GTCCACGAGGTCTCGCTCTTCCGATCTGCAGGTCGACATCTCGTAT	1100	0.23392907270515578	No Hit
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATAC	1063	0.22606054935052786	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCTCTCTCTTCCGATCTGCAGGTCGACATCTCGTAT	1047	0.22265794465663466	No Hit
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAA	1029	0.21883001437600483	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAAA	987	0.20989817705453526	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTACGCTCTTCCGATCTGCAGGTCGACATCTCGTATG	884	0.1879939093375979	No Hit
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA	870	0.1850166302304414	RNA PCR Primer, Index 41 (96% over 28bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTACC	868	0.18459130464370477	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCTCGTATGCCGTCTTCTGCTTGACGCTGCAGGTCG	860	0.18289000229675817	TruSeq Adapter, Index 21 (96% over 26bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA	852	0.18118869994981157	Illumina PCR Primer Index 11 (96% over 28bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAACC	848	0.1803380487763383	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGACGCTCTTCCGATCTGCAGGTCGACATCTCGTATGCC	839	0.17842408363602338	No Hit
GTCCACGCTCTTCCGATCTGCAGGTCGACATCTCGTATGCCGTCTT	825	0.17544680452886685	RNA PCR Primer, Index 41 (95% over 23bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAAA	781	0.16608964162066062	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA	766	0.16289969972013577	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAATC	759	0.16141106016655749	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	746	0.1586464438527693	No Hit
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATACC	740	0.15737046709255936	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAC	739	0.15715780429919102	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATCC	734	0.15609449033234943	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATCA	727	0.15460585077877115	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCACGCTCTTCCGATCTGCAGGTCGACATCTCGTAT	709	0.15077792049814132	No Hit
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAAA	702	0.14928928094456306	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGCA	699	0.14865129256445808	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATGCA	672	0.14290939714351336	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCTCCGCTCTTCCGATCTGCAGGTCGACATCTCGTA	665	0.1414207575899351	No Hit
GTCCACGAGGTCTCTACGCTGCAGGTCGACATCTCGTATGCCGTCT	656	0.1395067924496202	No Hit
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAT	649	0.1380181528960419	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCTCTACGCTCTTCCGATCTGCAGGTCGACATCTCG	633	0.13461554820214874	No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGCAAAAAA	629	0.13376489702867544	Illumina PCR Primer Index 11 (96% over 28bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACATCC	621	0.13206359468172885	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAATT	610	0.1297243039546773	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAGCA	568	0.12079246663320772	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAACGCTCTTCCGATCTGCAGGTCGACATCTCGTATGCCG	566	0.12036714104647107	No Hit
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACT	548	0.11653921076584124	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA	544	0.11568855959236796	RNA PCR Primer, Index 31 (96% over 31bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACATCA	537	0.11419992003878969	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAAC	512	0.10888335020458162	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGCTGCA	507	0.10782003623773999	RNA PCR Primer, Index 41 (96% over 30bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATTCC	504	0.10718204785763502	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCTTCCGATCTGCAGGTCGACATCTCGTATGCCGTC	500	0.10633139668416172	No Hit
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATTCA	491	0.10441743154384682	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCGCTCTTCCGATCTGCAGGTCGACATCTCGTATGC	490	0.10420476875047849	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTTT	20	0.0012550122	40.0	28
GCATAAT	45	1.2187229E-9	40.0	23
CTTGTTG	20	0.0012550122	40.0	28
CCCTGAA	20	0.0012550122	40.0	33
TCCGGAA	35	3.0512456E-7	40.0	15
TATTTTT	20	0.0012550122	40.0	31
ATTTATT	20	0.0012550122	40.0	27
ATTTAGG	25	7.787619E-5	40.0	18
CTTAAGA	40	1.924127E-8	40.0	14
ACAACGT	40	1.924127E-8	40.0	32
GTTGACT	30	4.861373E-6	40.0	13
TTTAGGG	25	7.787619E-5	40.0	19
CTTAAAC	55	5.456968E-12	40.0	22
TTGATAG	25	7.787619E-5	40.0	15
GTTTGCG	25	7.787619E-5	40.0	31
TGTTTAC	20	0.0012550122	40.0	30
CTTCGAG	35	3.0512456E-7	40.0	14
CAGAATC	30	4.861373E-6	40.0	37
AACGTCG	20	0.0012550122	40.0	20
AGCAATT	20	0.0012550122	40.0	23

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content