FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_14.3510000004f43f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_14.3510000004f43f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147534
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA141449.586942670841976Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC16141.0939851152954574No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA4860.32941559233803736No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG4490.3043366274892567No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA3720.2521452682093619RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG2530.1714858947767972No Hit
CTCAACGCCCTGCATACGAAAAGACAGAATCTCTTCCAAGAGCTTG2060.13962883132023807No Hit
GTCCACGAGGTCTCTATGACGTGTCGATCACTCCTCGTACGCTGCA1810.12268358480079168No Hit
GGAGTCGCCAGCGATAACCGGAGTAGTTGAAATGGTAATAAGACGA1780.12065015521845812No Hit
AAATTATCTTGATAAAGCAGGAATTACTACTGCTTGTTTACGAATT1760.11929453549690242No Hit
ATCATCTTCGGTTAAATCCAAAACGGCAGAAGCCTGAATGAGCTTA1640.11116081716756815No Hit
GTCCACGAGGTCTCTCTACGTCGGCTCATAGTCGTCGTACGCTGCA1620.10980519744601244No Hit
GTCCACGAGGTCTCTCAGACGAGCTAATTCTATCCCGTACGCTGCA1590.10777176786367888No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA1550.10506052842056747No Hit
GTCCACGAGGTCTCTAATGCACGGGCACCCTGTATCGTACGCTGCA1550.10506052842056747No Hit
GTCCACGAGGTCTCTGTGCGAACCAACGTACTACACGTACGCTGCA1530.10370490869901175No Hit
GTCCACGAGGTCTCTGGCCATGCCCAATATCAATACGTACGCTGCA1500.1016714791166782No Hit
GTCCACGAGGTCTCTGATAGATCCATCACGCTCCGTACGCTGCAGG1490.10099366925590034No Hit
GTCCACGAGGTCTCTAGATGTACCCAATTCTTCGACGTACGCTGCA1490.10099366925590034No Hit
GTCCACGAGGTCTCTAGCTGCACTGGATTCATAGTCGTACGCTGCA1480.10031585939512248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCCC950.040.00000413
TCTTATT950.040.00000413
TCTCCGG1450.040.00000413
CTCTACT1450.040.00000412
TCTATAG1450.040.00000413
TCCGGAG200.001252213840.015
TCCGGAA304.838679E-640.015
CCTTCGC200.001252213840.028
TCTGCCT1100.040.013
TATCATG451.20599E-940.015
GTGTTGC257.761616E-540.030
TCGCCAA200.001252213840.022
ATTTACC257.761616E-540.021
ACAACGC304.838679E-640.025
GTCATCT200.001252213840.024
GTTTGCG200.001252213840.031
CTATGTA304.838679E-640.014
GAGGATG304.838679E-640.023
GAGTGTA200.001252213840.033
GAAATGG200.001252213840.029
Produced by FastQC (version 0.11.2)