FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_13.3510000004f422.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_13.3510000004f422.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276050
Sequences flagged as poor quality0
Sequence length46
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA4490416.266618366238Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC30701.1121173700416591No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA11540.4180402101068647No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA11000.3984785364970114RNA PCR Primer, Index 41 (96% over 28bp)
GATTCAGCGAAACCAATCCGCGGCATTTAGTAGCGGTAAAGTTAGA7020.25430175692809276No Hit
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA6120.2216989675783373RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA3810.13801847491396485RNA PCR Primer, Index 41 (96% over 28bp)
GACTATTCCACTGCAACAACTGAACGGACTGGAAACACTGGTCATA3600.13041115739902193No Hit
GATTAGCGGCGTTGACAGATGTATCCATCTGAATGCAATGAAGAAA3480.12606411881905452No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA3200.11592102879913059No Hit
GTCCACGAGGTCTCTCAGACGAGCTAATTCTATCCCGTACGCTGCA3040.11012497735917406No Hit
GTCCACGAGGTCTCTGAATACTGCACAACGGCGCACGTACGCTGCA3020.1094004709291795No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA2970.10758920485419309Illumina PCR Primer Index 11 (96% over 28bp)
GTGACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAATGA2930.10614019199420395No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA2920.10577793877920667No Hit
GCAGAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTTCGGAT2870.10396667270422026No Hit
GTCCACGAGGTCTCTATTGCCGCGTGCATGGCTATCGTACGCTGCA2800.10143090019923927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCCC1850.040.00000413
GTGACTG2900.040.00000424
TATCCCT2900.040.00000416
ACGCGAA200.001254112540.026
CGGACAA257.7792545E-540.022
TCCGGCT304.854068E-640.015
TCCGGAA401.9192157E-840.015
CTTGTGT304.854068E-640.032
CCTTCCG900.040.031
ACAACTA200.001254112540.024
CTTGTCC200.001254112540.014
GTGTTGC353.0451338E-740.030
CTTAAGC650.040.014
CTTAAGA401.9192157E-840.014
TCGCCCG401.9192157E-840.031
TATTTCT304.854068E-640.016
TTCTATG304.854068E-640.015
TATTTCG200.001254112540.031
ACAACGC304.854068E-640.025
GTTGACG200.001254112540.031
Produced by FastQC (version 0.11.2)