FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_12.3510000004f415.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_12.3510000004f415.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136739
Sequences flagged as poor quality0
Sequence length46
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA3218223.535348364402257Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC14521.0618770065599425No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA8540.6245474955937955RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA4110.30057262375766974No Hit
CGCTTTAAAATAGTTGTTATAGATATTCAAATAACCCTGAAACAAA3280.2398730428041744No Hit
AGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTT3240.23694776179436738No Hit
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA2840.20769495169629731RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA2350.17186025932616153RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA2250.164547056801644Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGCAAAAAA1840.1345629264511222Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA1530.11189199862511792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTT200.001251892440.017
CTCTGGT850.040.012
CTTTTCA451.204171E-940.014
CTTTTAC257.758628E-540.014
GAGGCGT401.9070285E-840.032
AACGCTC200.001251892440.017
GAGGCCG200.001251892440.031
CAATGTT200.001251892440.026
GGAGACA200.001251892440.018
ACCGGCG257.758628E-540.031
TTGTTAT200.001251892440.014
GGTGAGT200.001251892440.019
CATGAGC200.001251892440.016
GTCATTG200.001251892440.024
TGCATAG200.001251892440.015
AACAAGC200.001251892440.024
GTTTGCG200.001251892440.031
TTGCAAA401.9070285E-840.037
ACTCGCA200.001251892440.022
TCCCACA200.001251892440.021
Produced by FastQC (version 0.11.2)