FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_11.3510000004f408.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_11.3510000004f408.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGGCGGAAAACGAACAAGCGCAAGAGTAAACATAGTGCCATGC35614.90787269681742No Hit
GTCGTAACCCAGCTTGGTAAGTTGGATTAAGCACTCCGTGGACAGA32113.442211055276381No Hit
TGCGTTGAGGCTTGCGTTTATGGTACGCTGGACTTTGTAGGATACC31213.06532663316583No Hit
TACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCCGCAACTTCGG29212.227805695142377No Hit
CAGCAATATCGGTATAAGTCAAAGCACCTTTAGCGTTAAGGTACTG24010.050251256281408No Hit
AAGGCTAATGATTCACACGCCGACTGCTATCAGTATTTTTGTGTGC1837.663316582914573No Hit
ATTGCATTCATCAAACGCTGAATAGCAAAGCCTCTACGCGATTTCA1466.113902847571189No Hit
CATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTATTGACG1466.113902847571189No Hit
CAGCGATAACCGGAGTAGTTGAAATGGTAATAAGACGACCAATCTG1375.7370184254606365No Hit
AAAGTAAGAGCTTCTCGAGCTGCGCAAGGATAGGTCGAATTTTCTC893.726968174204355No Hit
CGACTGAATGCCAGCAATCTCTTTTTGAGTCTCATTTTGCATCTCG833.47571189279732No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA40.16750418760469013Illumina PCR Primer Index 11 (96% over 28bp)
CAGCACTATCGGTATAAGTCAAAGCACCTTTAGCGTTAAGGTACTG30.12562814070351758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTG303.1076252E-640.00000437
ACTGCTA303.1076252E-640.00000423
ACACGCC303.1076252E-640.00000415
CAAGAAG303.1076252E-640.0000048
TTCACAC303.1076252E-640.00000412
ACGCCGA303.1076252E-640.00000417
CCGTGGA200.001017191640.00000436
TCCCAAG303.1076252E-640.0000045
AGCTTGG200.001017191640.00000411
CACACGC303.1076252E-640.00000414
CCCAGCT200.001017191640.0000048
CTTTAGC200.001017191640.00000428
ATAAGTC200.001017191640.00000414
CGTTAAG200.001017191640.00000434
CCAGCTT200.001017191640.0000049
AGAAGCT303.1076252E-640.00000410
ATGATTC303.1076252E-640.0000048
TAAGTTG200.001017191640.00000418
TGGTAAG200.001017191640.00000415
CAGTATT303.1076252E-640.00000431
Produced by FastQC (version 0.11.2)