000000000-AKRU9_l01n01_exp163gap1bff_10.3510000004f3fa.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_10.3510000004f3fa.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	73818
Sequences flagged as poor quality	0
Sequence length	46
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	3839	5.200628572976781	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	1195	1.6188463518383052	No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	290	0.3928581104879569	No Hit
AATATCCTTAAGAGGGCGTTCAGCAGCCAGCTTGCGGCAAAACTGC	187	0.25332574710775146	No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG	175	0.23706954943238778	No Hit
TTGCGTCTATTATGGAAAACACCAATCTTTCCAAGCAACAGCAGGT	173	0.23436018315316048	No Hit
TTTACCTTGCGTGTACGCGCAGGAAACACTGACGTTCTTACTGACG	171	0.2316508168739332	No Hit
AGTGGTCGGCAGATTGCGATAAACGGTCACATTAAATTTAACCTGA	154	0.20862120350050123	No Hit
GTCCACGAGGTCTCTAGCTGGCATCGACTCTATCTCGTACGCTGCA	124	0.16798070931209189	No Hit
ATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTC	122	0.16527134303286461	No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA	121	0.16391665989325097	No Hit
GTCCACGAGGTCTCTTACATACTAAGAGAGCCGCCCGTACGCTGCA	120	0.16256197675363732	No Hit
GTCCACGAGGTCTCTACAGTATGGACAGTTCCGCGCGTACGCTGCA	117	0.1584979273347964	No Hit
GTCCACGAGGTCTCTGATACTTTACCACAGCCTAGCGTACGCTGCA	99	0.1341136308217508	No Hit
GTCCACGAGGTCTCTGAATACTGCACAACGGCGCACGTACGCTGCA	98	0.13275894768213717	No Hit
GTCCACGAGGTCTCTGCGATCAGAACAGATGCCGACGTACGCTGCA	94	0.12734021512368257	No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA	93	0.12598553198406892	No Hit
GTCCACGAGGTCTCTCGAGTAAGTAGAGCTGATTCCGTACGCTGCA	91	0.12327616570484162	No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA	91	0.12327616570484162	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTGATAGATCCATCACGCTCCGTACGCTGCAGG	90	0.121921482565228	No Hit
GTCCACGAGGTCTCTGAAGAGTTCAAGCCATCCCCGTACGCTGCAG	90	0.121921482565228	No Hit
GTCCACGAGGTCTCTGGCTAGAACCAATTCCCAGACGTACGCTGCA	90	0.121921482565228	No Hit
GTCCACGAGGTCTCTTAAGATGTGTGAACTGTGTCCGTACGCTGCA	88	0.11921211628600072	No Hit
GTCCACGAGGTCTCTACGAACGTGGCATGTTTGATCGTACGCTGCA	87	0.11785743314638707	No Hit
GTCCACGAGGTCTCTTCATGGCGTTAATGGCTATGCGTACGCTGCA	84	0.11379338372754612	No Hit
GTCCACGAGGTCTCTATTATAGTGCCAGACGTACGCTGCAGGTCGA	83	0.1124387005879325	No Hit
GTCCACGAGGTCTCTTACTGAGCGGCATGTCACTGCGTACGCTGCA	82	0.11108401744831883	No Hit
GTCCACGAGGTCTCTTACTGCTCGATAGGTGGATTCGTACGCTGCA	82	0.11108401744831883	No Hit
GTCCACGAGGTCTCTAGTATCTCACTACGGCTCATCGTACGCTGCA	82	0.11108401744831883	No Hit
GTCCACGAGGTCTCTAGCGCAGGCCAATGGTTATACGTACGCTGCA	81	0.10972933430870518	No Hit
GTCCACGAGGTCTCTAGGCGCGTGCTATATCTCTTCGTACGCTGCA	81	0.10972933430870518	No Hit
GTCCACGAGGTCTCTTGTGTGTCAACAACCACCAACGTACGCTGCA	80	0.10837465116909156	No Hit
GTCCACGAGGTCTCTTCGACAGCAAGACGATCTGACGTACGCTGCA	79	0.1070199680294779	No Hit
GTCCACGAGGTCTCTACCTTACAGTTTACAGAGGGCGTACGCTGCA	79	0.1070199680294779	No Hit
GTCCACGAGGTCTCTAGATGTACCCAATTCTTCGACGTACGCTGCA	78	0.10566528488986425	No Hit
GTCCACGAGGTCTCTCACTATACGTTGAGACCATGCGTACGCTGCA	78	0.10566528488986425	No Hit
GTCCACGAGGTCTCTGAATATGCGCTCCCACTGTGCGTACGCTGCA	78	0.10566528488986425	No Hit
GTCCACGAGGTCTCTCTTGACAGGAGTGATAAACCGTACGCTGCAG	76	0.10295591861063697	No Hit
GTCCACGAGGTCTCTAGGGACCAGTCACAGTCATCCGTACGCTGCA	74	0.10024655233140968	No Hit
GTCCACGAGGTCTCTACACCAGGGAAGGGCTTCACGTACGCTGCAG	74	0.10024655233140968	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGAAT	20	0.0012481497	40.000004	22
ATCCCGA	20	0.0012481497	40.000004	29
CTTTCCC	20	0.0012481497	40.000004	14
CTCTGGT	120	0.0	40.000004	12
ACCGGTC	20	0.0012481497	40.000004	26
ATCCTCA	20	0.0012481497	40.000004	20
AGCCTCA	20	0.0012481497	40.000004	19
CTCTGAA	120	0.0	40.000004	12
TGCACTC	20	0.0012481497	40.000004	15
CCAACTT	20	0.0012481497	40.000004	24
TCCTCAG	20	0.0012481497	40.000004	21
CAGTCTT	20	0.0012481497	40.000004	25
AAACTCT	20	0.0012481497	40.000004	25
CCAATGC	20	0.0012481497	40.000004	24
AATCTGT	20	0.0012481497	40.000004	27
GTGTTCG	20	0.0012481497	40.000004	31
GGCATGT	30	4.8058027E-6	40.000004	24
GCCTCAC	20	0.0012481497	40.000004	20
CTTTATG	30	4.8058027E-6	40.000004	14
CTTTATC	20	0.0012481497	40.000004	14

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content