FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_1.3510000004f366.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_1.3510000004f366.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4442
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTACCATACTCAGGCACACAAAAATACTGATAGCAGTCGGCGTG4419.927960378208015No Hit
GGTTAGCCTCGGTACGGTCAGGCATCCACGGCGCTTTAAAATAGTT4099.20756416028816No Hit
AGCCTTGAATGGCAGATTTAATACCAGCATCACCCATGCCTACAGT3537.946870778928411No Hit
CGGCGTTGACAGATGTATCCATCTGAATGCAATGAAGAAAACCACC3427.69923457901846No Hit
GGTCAACGCTACCTGTAGGAAGTGTCCGCATAAAGTGCACCGCATG3377.586672669968483No Hit
CATAAGCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAGAAGT2916.55110310670869No Hit
AGCGCATGACAAGTAAAGGACGGTTGTCAGCGTCATAAGAGGTTTT2786.2584421431787485No Hit
CCTTGCGACCCTCGGCAGCAAGAACCATACGACCAATATCACGAAA2515.65060783430887No Hit
AGGCGTTTTATGATAATCCCAATGCTTTGCGTGACTATTTTCGTGA2495.605583070688879No Hit
AAATATCCTTTGCAGTAGCGCCAATATGAGAAGAGCCATACCGCTG2395.380459252588924No Hit
AGCCAATACCATCAGCTTTACCGTCTTTCCAGAAATTGTTCCAAGT2325.222872579918955No Hit
ACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGCAATTAAAA2064.637550652859073No Hit
TGGTTGAACAGCATCGGACTCAGATAGTAATCCACGCTCTTTTAAA1994.479963980189104No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA80.18009905447996397Illumina PCR Primer Index 11 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCCT200.001124374340.00000430
TTATGAT401.2883902E-840.0000048
ATCCTCA200.001124374340.00000410
ATCCCAA401.2883902E-840.00000416
AGGGGCC200.001124374340.00000421
TCCTCAA200.001124374340.00000411
CAATGCT401.2883902E-840.00000420
AAGTAAG200.001124374340.00000416
CAATGAA200.001124374340.00000430
TCAAGTA200.001124374340.00000414
CCAATGC401.2883902E-840.00000419
AATCCCA401.2883902E-840.00000415
TCCCAAT401.2883902E-840.00000417
TGCAATT200.001124374340.00000436
TTCGTGA401.2883902E-840.00000440
TGCAATG200.001124374340.00000428
TGCGTGA401.2883902E-840.00000428
GGCGTTT401.2883902E-840.0000042
TGACTAT401.2883902E-840.00000432
AATTTAT200.001124374340.0000045
Produced by FastQC (version 0.11.2)