000000000-AKRU9_l01n01.3310000004f318.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01.3310000004f318.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23752625
Sequences flagged as poor quality	0
Sequence length	51
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCGCTGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	371769	1.5651701654027714	RNA PCR Primer, Index 41 (96% over 30bp)
TCATTGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	198510	0.8357392077717727	RNA PCR Primer, Index 11 (96% over 28bp)
ATACCGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	164840	0.6939864541287543	RNA PCR Primer, Index 41 (96% over 30bp)
CCTTAGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	132690	0.558632993195489	RNA PCR Primer, Index 41 (96% over 30bp)
GCAAGGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	113414	0.4774798574894354	Illumina PCR Primer Index 11 (96% over 28bp)
TCTAGGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	100145	0.42161655816988647	RNA PCR Primer, Index 11 (96% over 28bp)
TAGCCGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	96094	0.4045616010861957	RNA PCR Primer, Index 11 (96% over 28bp)
CCTACGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	87886	0.37000542045352885	RNA PCR Primer, Index 41 (96% over 30bp)
GCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA	86480	0.36408607469700716	RNA PCR Primer, Index 41 (96% over 30bp)
CCACAGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	64128	0.26998279137569003	TruSeq Adapter, Index 11 (96% over 28bp)
TACGCGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	59260	0.249488214460507	RNA PCR Primer, Index 11 (96% over 28bp)
GTCCTGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	51788	0.21803063871887846	Illumina PCR Primer Index 11 (96% over 28bp)
GCGGTGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	48767	0.2053120444582441	Illumina PCR Primer Index 11 (96% over 28bp)
ATGCGGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	47879	0.20157351029623044	RNA PCR Primer, Index 41 (96% over 30bp)
GATCAGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	45324	0.1908168044584546	Illumina PCR Primer Index 11 (96% over 28bp)
AACCTGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	44904	0.18904857884128595	RNA PCR Primer, Index 41 (96% over 30bp)
ACTCAGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	41339	0.1740397114003189	RNA PCR Primer, Index 41 (96% over 30bp)
GCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAC	38566	0.1623652122660127	RNA PCR Primer, Index 41 (96% over 30bp)
CACGGGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	32182	0.13548818288504955	RNA PCR Primer, Index 41 (96% over 30bp)
GCGCAGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	28407	0.1195952026354982	Illumina PCR Primer Index 11 (96% over 28bp)
GTGCCGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	28404	0.1195825724525184	RNA PCR Primer, Index 41 (96% over 30bp)
CGCTTGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	27327	0.11504833676277886	TruSeq Adapter, Index 11 (96% over 28bp)
CCAATGTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	26953	0.11347377395130012	RNA PCR Primer, Index 41 (96% over 30bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGAGTC	35	1.2132841E-7	45.000004	30
TGGAATT	525	0.0	45.0	28
GTGGGCT	570	0.0	45.0	34
GGGAGAT	515	0.0	45.0	34
CGGGGTG	25	3.8931314E-5	45.0	34
GATGGGT	370	0.0	45.0	22
GGTTGGG	625	0.0	45.0	28
AAGTGGG	160	0.0	45.0	27
GGGATGG	40	6.8248482E-9	45.0	30
GCGGTGT	38250	0.0	44.964706	1
TGCGAAA	1460	0.0	44.84589	26
CGCTGTC	53685	0.0	44.819782	2
GCGGGTC	27280	0.0	44.81855	2
AGACGTC	27500	0.0	44.754543	2
TCCACGA	1998155	0.0	44.74608	7
GGTGTTG	820	0.0	44.725605	27
CCGTCCA	334630	0.0	44.714237	4
GGATCCG	3115	0.0	44.711075	36
CCACGAG	1996160	0.0	44.702427	8
ACGAGGT	1993235	0.0	44.69138	10

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content