FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_plasmid_mix.3520000003f853.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_plasmid_mix.3520000003f853.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320466
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGGAGGGCGTCCCCGGCCCGGCGCTGCTCCCGCGTGTGTCCTGGGG3263210.182671484650479No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT140444.3823681763432Illumina Single End PCR Primer 1 (96% over 32bp)
GGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAACAAAG31420.9804472237304426No Hit
GGCCGCGCTCCCCTCGCGCACACTGCGGAGCGCACAAAGCCCCGCGCCGC18180.5672988710190785No Hit
GGCCGCCCGTGCCACACGCAGGGGCCGGCCCGTCTAGAATTGACCTGCAT14260.44497700224048725No Hit
GCCGGGAGGGCGTCCCCGGCCCGGCGCTGCTCCCGCGTGTGTCCTGGGGT3630.11327254685364438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGT35200.0279.914768
GGGCGTC36050.0274.951489
GAGGGCG39050.0253.072987
GGAGGGC44600.0222.572866
GGCGGTG6950.0186.762598
GCCCGTG2750.0182.363635
GGGAGGG55200.0179.2985
GCCGGGA55650.0176.522922
CGGGAGG56550.0174.235184
CCGGGAG58700.0168.85863
GGCCGGG69550.0159.270311
CGCCCGT3650.0137.397264
CGTGCCA3700.0135.540548
CCCGTGC4150.0124.397586
GGGCGGT11150.0115.089687
AGGCCGT559.526551E-6107.272728
CGGGGGC13350.098.3333364
CGCGCTC5300.097.4056554
GGGGGCG14500.091.551735
CCGCGCT5400.090.138893
Produced by FastQC (version 0.11.2)