FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b8.3520000003f4b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b8.3520000003f4b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196576
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT34091.7341893211785773No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA33441.7011232296923327No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA32581.6573742471105324No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA31221.588189809539313No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA30521.5525801725541266No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC29471.499165717076347No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT22601.149682565521732No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG19971.015892072277389No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT18560.9441640892072276No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT15500.7884991046719845No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT13730.6984575940094416No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT12380.6297818655380107No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT12020.6114683379456292No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC11920.6063812469477454No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT10740.5463535731727168No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA9700.4934478267947257No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG9140.4649601172065766No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT8350.4247720983232948No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT7430.3779708611427641No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA7310.3718663519453036No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5840.2970861142764122No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTA5430.27622904118508873No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTCT5420.27572033208530033No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA5260.2675809864886863No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT4880.24825004069672796No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT4560.23197134950349993No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG3870.19687042161810192No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA3870.19687042161810192No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC3670.18669623962233436No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG3450.17550463942699007No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT3340.1699088393293179No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC2950.15006918443757122No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC2710.13786016604265017No Hit
GGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTAT2340.11903792935048021No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTATT2210.11242471105323132No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG2130.10835503825492432No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATCTATTTTGACAAGA2120.10784632915513592No Hit
GGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACAA2030.10326794725704054No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT26050.0291.036471
CGGGTTA26150.0289.359472
GGGGGGA24300.0273.755161
TATTAGT28200.0267.801427
ATTAGTA28500.0265.499978
TTATTAG29100.0259.012026
GTTATTA29250.0257.17955
TTAGTAG29850.0253.492469
GGGGAGC26900.0247.295553
GGAGCAG27450.0242.877965
GGGTTAT31800.0237.484283
GAGCAGA28000.0236.52686
CAGAAGC28150.0235.79049
GGTTATT33250.0227.127844
GGGGGAG29450.0225.882862
GGGAGCA30200.0220.273184
GCAGAAG32100.0207.23528
AACGCCC150.0028479071196.66667295
AGCAGAA34800.0189.885067
GCGCTTT200.006733477147.5295
Produced by FastQC (version 0.11.2)