FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b7.3520000003f4a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b7.3520000003f4a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179135
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAGCAATCAAGTTCAG35631.9890027074552712No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA32701.8254389147849388No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC20621.1510871688949673No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC19921.1120104948781646No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA17000.949004940408072No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT15930.8892734529823875No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA14170.7910235297401402No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT12850.7173360873084544No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTTGTTCTTTATAT11040.6162949730650068No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT9670.5398163396321211No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT9440.5269768610266No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA8460.47226951740307593No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTTCATTTTAATCATTTGTT8390.46836185000139563No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT7270.4058391715745109No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTTGTTCTTTATAT7010.39132497836826974No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG6040.33717587294498563No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5770.32210344153850445No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC5260.2936332933262623No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAGCAATCAAGTTCAG4920.2746531945181009No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA4910.27409495631786085No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA4350.24283361710441845No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC4140.23111061489937754No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC4060.2266447092974572No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT3940.21994585089457672No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTTCATTTTAATCATTTGTT3770.210455801490496No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTT3460.19315041728305468No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2930.16356379267033241No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGAGAGAAAACTAAAGAAAATGC2910.16244731626985234No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA2720.15184079046529153No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG2680.14960783766433136No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA2630.14681664666313116No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA2540.14179250286097078No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTCATT2260.1261618332542496No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG2210.12337064225304936No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT2120.118346498450889No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT19250.0294.233761
CGGGTTA19850.0286.82622
GGGGGGA18450.0259.024381
TTATTAG22100.0257.624426
TATTAGT22300.0255.975367
ATTAGTA22350.0255.402688
GGGTTAT22250.0255.224723
GGGGAGC18850.0252.745353
GGAGCAG18950.0250.633245
GAGCAGA19450.0244.190226
GTTATTA24400.0233.340165
TTAGTAG24400.0232.735669
GGTTATT24900.0228.654634
GGGAGCA20950.0227.41054
CAGAAGC23950.0198.308999
GCCCGAC150.0028476974196.66666295
GGGGGAG24950.0192.134262
GCAGAAG25150.0188.260448
AGCAGAA30150.0157.03987
GGTATTG200.0067329817147.5295
Produced by FastQC (version 0.11.2)