FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b56.3520000003f81f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b56.3520000003f81f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172348
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA52463.0438415299278203No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG32181.8671525053960591No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA27061.5700791422006637No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA24091.3977533826908348No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC22681.315942163529603No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA22431.3014366282173278No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA17150.9950797224220762No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT15650.9080465105484252No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT12060.699747023464154No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA12000.6962656949892079No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGT10230.5935665049782997No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACA9910.5749994197785875No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT9350.5425070206790912No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT9310.540186135029127No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGA8210.4763617796551164No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG8080.46881890129273335No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGGTTCTTGTTTCTTTTTAT8030.4659177942302783No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTG6090.353354840207023No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT6060.35161417596954997No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA5690.33014598370738274No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTGT5110.29649314178290437No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA5000.2901107062455033No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGGTTCTTGTTTCTTTTTAT4960.28778982059553926No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA4710.2732842852832641No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGT4330.2512358716086059No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT3800.22048413674658252No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG3320.19263350894701417No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT3300.19147306612203216No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAA3290.19089284470954115No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATGAGA3120.18102908069719403No Hit
CCGGGTTATTAGTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGT3110.18044885928470306No Hit
GGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAA3040.176387309397266No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAA2790.16188177408499083No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAA2710.15724000278506278No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT2180.12648826792303944Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAGAAGCAGAGCCATGGATGAACTCCACAACGAAATACTAGA2020.11720472532318334No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT1910.11082228978578225No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT1900.11024206837329126No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTGT1760.10211896859841717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT22950.0292.42921
CGGGTTA23400.0286.805542
GGGGGGA21050.0285.190031
GTTATTA23800.0282.605045
GGGTTAT24650.0272.860053
TTATTAG25600.0263.886756
TATTAGT25850.0260.193427
TTAGTAG26600.0253.411679
ATTAGTA26850.0251.052158
GGGGAGC24150.0247.360263
GGTTATT27450.0245.564654
GGAGCAG25300.0237.282615
GAGCAGA25450.0235.304526
CAGAAGC25650.0234.044839
GGGGGAG26650.0225.262662
GGGAGCA28300.0211.607774
GCAGAAG28250.0211.460178
AGCAGAA28550.0209.75487
CGGTTTA200.00673276147.500022
CCAGGTT2200.0100.56818295
Produced by FastQC (version 0.11.2)