FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b55.3520000003f802.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b55.3520000003f802.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198344
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTTTTGTAAT31921.6093252127616666No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA30271.526136409470415No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG28041.4137054813858752No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC21971.1076715201871496No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA21691.0935546323559069No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC18840.9498648812164725No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT16940.8540717137901827No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA16760.8449965716129554No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT14030.707356915258339No Hit
GGGGGGAGCAGAAGCGGTGCATTTGATTTGTCATAATGGATACTTTTATT12370.6236639374016859No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG11850.5974468600008067No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT11470.5782882265155488No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT11450.5772798773847456No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTTTTGTAAT11390.5742548299923366No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT10480.5283749445407978No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTGTAT10070.5077037873593353No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC9970.5026620417053201No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT8510.42905255515669743No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGAGAGAAAACTAAAGAAAATGC7750.39073528818618153No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC6610.3332593877304078No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT6560.33073851490340017No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC6470.3262009438147864No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTGTAT6350.32015084902996815No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG5060.25511233009317147No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT4490.22637437986528455No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT4100.20671157181462507No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA4050.20419069898761746No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC3740.1885612874601702No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA3510.17696527245593513No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA3100.15629411527447262No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG2910.14671479853184366No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG2860.14419392570483605No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT2750.13864800548541925No Hit
GAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACAAAATAGACAC2450.12352276852337354No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA2400.12100189569636591No Hit
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT2180.10991005525753236No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT2060.10385996047271406No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT2050.10335578590731254Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT23900.0292.53141
CGGGTTA24150.0289.503082
GGGGGGA21050.0264.869351
GGGTTAT26800.0261.97763
TTATTAG27350.0257.248636
TATTAGT27500.0255.845447
ATTAGTA28050.0250.828878
GGGGAGC23200.0240.959033
TTAGTAG29150.0240.351629
GGTTATT29250.0239.529924
GTTATTA29950.0234.424045
GGAGCAG24050.0231.216225
CAGAAGC24250.0227.484539
GAGCAGA24750.0224.080816
GGGAGCA26450.0211.35164
GGGGGAG27700.0201.28162
GCAGAAG30550.0181.538458
AGCAGAA35550.0155.590737
CGAGGGG301.147655E-4147.5295
GTCAGGG352.120425E-4126.428566295
Produced by FastQC (version 0.11.2)