FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b54.3520000003f7f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b54.3520000003f7f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281276
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA84242.994923135994539No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA77422.752456661784155No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC73162.6010039960750295No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT70822.517811686741848Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT39311.3975596922595601No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA23370.8308565252634422No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA21930.7796612579814844No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAATTACTAGA21020.7473086932408026No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT20900.7430424209673061No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG18570.6602056343235826No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA17800.6328303872353134No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTG17440.6200315704148238No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA16430.5841237787795617No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT16330.5805685518849812No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTTAT15370.5464383736970093No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAA14220.5055532644093346No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACA14010.4980872879307157No Hit
GGGGGGAGGGAAAAATCTGTGTACTTGTACTGCCGAGTGAATGGCACAAA13990.49737624255179963No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTG11690.4156060239764502No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGT10250.36441075669449224No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT10190.362277620557744No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG9340.3320581919538105No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGTTTCTTTTTAT9320.33134714657489445No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGT6990.24851035993117082No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG6880.24459961034713237No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTGT5800.2062031598856639No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT5410.1923377749968003No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATGAGA5110.18167209431305906No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAA5100.181316571623601No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA5060.17989448086576887No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA5020.1784723901079367No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAATTACTAGA4610.163895959840157No Hit
AGAAGCGGTGCCATGGATGAACTCCACAACGAATTACTAGAACTAGATGA4490.15962968756666052No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA4420.1571410287404542No Hit
AAGCGGTGCCATGGATGAACTCCACAACGAATTACTAGAACTAGATGAGA4330.15394132453533185No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT3980.1414980304043004No Hit
GAAGCGGTGCCATGGATGAACTCCACAACGAATTACTAGAACTAGATGAG3920.13936489426755216No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCACTCAATTGCGTTCATTG3350.11910010096844381No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAAT3340.11874457827898577No Hit
CAGAAGCGGTGCCATGGATGAACTCCACAACGAATTACTAGAACTAGATG2870.10203501187445783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAT255.311449E-10295.0295
CCGGGTT33750.0290.19261
CGGGTTA34150.0285.929722
GGGTTAT34650.0280.95243
GGGGGGA35050.0279.850221
TATTAGT37400.0262.266057
ATTAGTA38100.0257.06048
TTAGTAG38250.0256.052289
GGTTATT38750.0250.845174
GTTATTA38900.0249.87795
GGGGAGC37400.0247.27943
TTATTAG39700.0246.32876
GGAGCAG39450.0233.681875
GGGGGAG42450.0231.065952
GAGCAGA40700.0225.780096
CAGAAGC42050.0217.128439
AGCAGAA42950.0213.952297
GCAGAAG46200.0198.582268
GGGAGCA50350.0183.38634
GCTCGAC553.1468517E-10160.9091295
Produced by FastQC (version 0.11.2)