FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b53.3520000003f7e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b53.3520000003f7e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217387
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA36091.660172871422854No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA29671.3648470239710746No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG29401.3524267780502053No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC29191.3427665867784182No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA26961.240184555654202No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC21720.9991397829677028No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA21170.9738392820177839No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT18740.8620570687299609No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT13740.6320525146397898No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT13590.6251523780170848No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT12410.5708713032518044No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT10330.47518940875029325No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA9150.4209083339850129No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT8800.40480801519870097No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT8770.40342798787415995No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG8040.36984732297699496No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT7080.3256864485916821No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG6750.3105061480217308No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAACAAGA6610.30406602050720605No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC6440.2962458656681402No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC5330.24518485466012227No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT5240.24104477268649918No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT4620.21252420797931798No Hit
CCGGGTTATTAGTAGTAACAAGAGCAGTACTCAAACAAATGAGAGACCAA4130.18998376167848124No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT4040.18584367970485816No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG3980.1830836250557761No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA3970.18262361594759577No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT3860.17756351575761198No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC3670.16882334270218552No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC3550.16330323340402142No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGAGAGAAAACTAAAGAAAATGC3470.15962316053857867No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA2650.12190241366779062No Hit
CCGGGTTATTAGTAGAAACAACAGCCTACTCATTCAACCAATAGCCCTGG2400.11040218596328208No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTCTGGGTAACAGTGAACACGGGGA2360.1085621495305607No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA2250.10350204934057694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT25150.0290.894621
CGGGTTA26050.0280.278322
GGGGGGA22250.0258.539341
TATTAGT28800.0254.539927
TTATTAG28850.0253.587516
GGGGAGC22850.0251.105043
GAGCAGA23300.0246.255376
ATTAGTA29800.0245.503368
GGGTTAT29800.0245.503363
GGAGCAG23450.0244.05125
CAGAAGC23950.0238.956159
TTAGTAG30850.0237.625639
GTTATTA31550.0231.88595
GGGAGCA26300.0218.726234
GGTTATT33900.0215.811224
GGGGGAG27800.0206.924472
GCAGAAG28700.0199.92168
CGGGCTA150.0028481137196.666672
AGCAGAA31250.0184.087
GCTCGAT652.1823473E-590.76923295
Produced by FastQC (version 0.11.2)