FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b52.3520000003f7da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b52.3520000003f7da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197103
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG27381.3891214238240919No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA26811.360202533700654No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC24821.259240092743388No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA23971.216115432032998No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT21311.0811606114569539No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCA19470.9878084047426979No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT16680.846258047822712No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT15490.7858835228281659No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA15230.772692450140282No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT14660.743773560016844No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT13000.6595536343942No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT12150.61642897368381No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT11020.5590985423864681No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT10650.5403266312537099No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG9960.505319553735864No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT9940.504304855836796No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT9790.49669462159378597No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA9460.47995210625916396No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA8890.451033216135726No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG8740.443422981892716No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA7450.37797496740283No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT6270.31810779135781797No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCA6020.305424067619468No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5680.28817420333531196No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA5360.271939036950224No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG5260.266865547454884No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCA4310.21866739724915402No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT4170.211564511955678No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC3660.185689715529444No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT3260.165395757548084No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG3120.158292872254608No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTCT2960.150175289062064No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTA2920.148145893263928No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA2730.138506263222782No Hit
CCGGGTTATTAGTAGTAACAAGAGCAGTACTCAAACAAATGAGAGACCAA2690.13647686742464601No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAA2660.134954820576044No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT2010.101977138856334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT21000.0294.29761
CGGGTTA21950.0281.560362
GGGGGGA21350.0263.220121
TATTAGT25250.0244.178227
TTATTAG25250.0243.594076
GGGGAGC23050.0242.52713
GTTATTA25500.0241.205895
ATTAGTA25550.0240.733868
GGAGCAG23300.0240.557945
GGGTTAT25950.0238.159933
GAGCAGA23650.0235.750526
CAGAAGC23800.0235.504219
GGTTATT27050.0227.929764
TTAGTAG27150.0226.546989
GGGAGCA25550.0219.373784
GGGGGAG27300.0205.311362
GCAGAAG28350.0197.707238
AGCAGAA30850.0180.25127
CCGACAA508.759929E-488.5295
TCGGTTT650.002481697168.07693295
Produced by FastQC (version 0.11.2)